The relationship of glycerol and glycolysis metabolism patway under hyperosmotic stress in Dunaliella salina
| Autor: | Bing-Bing Xia, Shuang-Hui Wang, Lin-Han Bai, Jing-Bo Duan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
General Immunology and Microbiology
biology Osmotic shock QH301-705.5 General Neuroscience dunaliella salina Metabolism cooperative regulation glycolysis biology.organism_classification General Biochemistry Genetics and Molecular Biology Enzyme assay enolase Citric acid cycle chemistry.chemical_compound chemistry Biochemistry phosphofructokinase Dunaliella salina biology.protein Glycerol Glycolysis Biology (General) General Agricultural and Biological Sciences Phosphofructokinase |
| Zdroj: | Open Life Sciences, Vol 9, Iss 9, Pp 901-908 (2014) |
| ISSN: | 2391-5412 |
| Popis: | This study detected the upstream and downstream key genes of glycolysis in Dunaliella Salina by using Real-time Fluorescence Quantitative PCR assays and measurement of enzyme activity. The results were as follows: the levels of transcription, enzyme activity, and protein of D. salina PFK were up-regulated under hyperosmotic stress while D. salina ENO were down-regulated. At the same time we monitored the change of intracellular degradation of starch, the synthesis of glycerol and PEP concentration in Dunaliella Salina under hyperosmotic stress. We found that lower expression of DsENO reduced the concentration of intracellular PEP which promoted the degradation of starch, and decreased the flow of carbon into the tricarboxylic acid cycle which would favor the synthesis of glycerol. |
| Databáze: | OpenAIRE |
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