A vaccinia-based single vector construct multi-pathogen vaccine protects against both Zika and chikungunya viruses
| Autor: | Kexin Yan, Alexander A. Khromykh, Jody Hobson-Peters, Bing Tang, Yin Xiang Setoh, Natalie A. Prow, Jessamine E. Hazlewood, Liang Liu, Thuy T. Le, John D. Hayball, Kerrilyn R. Diener, Tamara H. Cooper, Preethi Eldi, Andreas Suhrbier, Eri Nakayama, Paul Howley |
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| Přispěvatelé: | Prow, Natalie A, Liu, Liang, Nakayama, Eri, Cooper, Tamara H, Yan, Kexin, Eldi, Preethi, Hazlewood, Jessamine E, Tang, Bing, Le, Thuy T, Setoh, Yin Xiang, Khromykh, Alexander A, Hobson-Peters, Jody, Diener, Kerrylin R, Howley, Paul M, Hayball, John D, Suhrbier, Andreas |
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine viruses General Physics and Astronomy Sementis Copenhagen Vector Receptor Interferon alpha-beta medicine.disease_cause Zika virus chemistry.chemical_compound Pregnancy Chlorocebus aethiops Chikungunya lcsh:Science Maternal-Fetal Exchange Multidisciplinary biology Zika Virus Infection Viral Vaccine virus diseases Female Chikungunya virus Aedes albopictus Science Genetic Vectors Enzyme-Linked Immunosorbent Assay Vaccinia virus Viremia CHO Cells Aedes aegypti Article General Biochemistry Genetics and Molecular Biology Virus 03 medical and health sciences Cricetulus parasitic diseases medicine Animals Humans Vero Cells chikungunya virus Viral Vaccines Zika Virus General Chemistry vaccination biology.organism_classification medicine.disease Antibodies Neutralizing Virology Mice Inbred C57BL 030104 developmental biology chemistry Chikungunya Fever lcsh:Q Vaccinia HeLa Cells |
| Zdroj: | Nature Communications, Vol 9, Iss 1, Pp 1-12 (2018) Nature Communications |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-018-03662-6 |
| Popis: | Zika and chikungunya viruses have caused major epidemics and are transmitted by Aedes aegypti and/or Aedes albopictus mosquitoes. The “Sementis Copenhagen Vector” (SCV) system is a recently developed vaccinia-based, multiplication-defective, vaccine vector technology that allows manufacture in modified CHO cells. Herein we describe a single-vector construct SCV vaccine that encodes the structural polyprotein cassettes of both Zika and chikungunya viruses from different loci. A single vaccination of mice induces neutralizing antibodies to both viruses in wild-type and IFNAR−/− mice and protects against (i) chikungunya virus viremia and arthritis in wild-type mice, (ii) Zika virus viremia and fetal/placental infection in female IFNAR−/− mice, and (iii) Zika virus viremia and testes infection and pathology in male IFNAR−/− mice. To our knowledge this represents the first single-vector construct, multi-pathogen vaccine encoding large polyproteins, and offers both simplified manufacturing and formulation, and reduced “shot burden” for these often co-circulating arboviruses. Zika and chikungunya virus are co-circulating in many regions and currently there is no approved vaccine for either virus. Here, the authors engineer one vaccinia virus based vaccine for both, Zika and chikungunya, and show protection from infection and pathogenesis in mice. |
| Databáze: | OpenAIRE |
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