Expression of apoptosis regulatory proteins of the Bcl-2 family and p53 in primary resected non-small-cell lung cancer
| Autor: | Markus Borner, John C. Reed, Michel A. Hotz, D Schlaifer, H. J. Altermatt, S Vonlanthen, M Bacchi, B Pfanner-Meyer, Pierre Brousset, Daniel C. Betticher |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Male
p53 Cancer Research Lung Neoplasms Tumor suppressor gene Population Apoptosis Disease-Free Survival Bcl-2-associated X protein Carcinoma Non-Small-Cell Lung Proto-Oncogene Proteins medicine Humans Bcl-2 Lung cancer education Neoplasm Staging Retrospective Studies bcl-2-Associated X Protein education.field_of_study biology Bcl-2 family Bak Membrane Proteins Mcl-1 Cancer Regular Article medicine.disease Immunohistochemistry Neoplasm Proteins Survival Rate bcl-2 Homologous Antagonist-Killer Protein Proto-Oncogene Proteins c-bcl-2 non-small-cell lung cancer Oncology Membrane protein Bax Cancer research biology.protein Myeloid Cell Leukemia Sequence 1 Protein Female Tumor Suppressor Protein p53 Bcl-2 Homologous Antagonist-Killer Protein |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| DOI: | 10.1038/sj.bjc.6690152 |
| Popis: | Proteins of the Bcl-2 family as well as p53 are important regulators of apoptosis. Alterations in the expression of these proteins can contribute to the formation of cancer, as well as influence tumour response to chemo- and radiotherapy. We used antibodies specific for the human Bcl-2, Mcl-1, Bax, Bak and p53 proteins to examine the expression of these apoptosis-regulating genes in 49 archival specimens of patients with radically resected non-small-cell lung cancer (NSCLC). Tumour cells containing immunostaining for the antiapoptotic proteins Bcl-2 and Mcl-1 were present in 31% and 58% of the cases evaluated, respectively, whereas immunopositivity for the proapoptotic proteins Bax and Bak was found in 47% and 58% of the samples. p53 immunopositivity was detected in 61% of the samples. The expression of Bcl-2 and p53 and the expression of Mcl-1 and Bax showed a positive association (P= 0.02 and P= 0.06 respectively), whereas the expression of Bax was inversely related to p53 (P= 0.008). The expression of Bcl-2 had a negative influence on relapse-free survival in this population of primary resected NSCLC patients (P= 0.02). The expression of p53 and Bcl-2 was significantly associated with metastasis-free survival (P< 0.01). Only patients with p53-positive tumours developed metastases during the follow-up period. Our results establish the frequent expression of the Bcl-2 family proteins Bcl-2, Mcl-1, Bax and Bak in NSCLC. It can be expected that Bcl-2 family members have no straightforward impact on clinical outcome in this disease because their interactions in the regulation of apoptosis are complex. © 1999 Cancer Research Campaign |
| Databáze: | OpenAIRE |
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