Human ureaplasmas show diverse genome sizes by pulsed-field electrophoresis
| Autor: | Gerald W. Stemke, Louise E. Pyle, Janet A. Robertson, Lloyd R. Finch |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
DNA
Bacterial Electrophoresis Agar Gel Genetics biology Restriction Mapping Mycoplasmataceae Mycoplasma hominis biology.organism_classification Ureaplasma Molecular biology Molecular Weight Acholeplasma Mycoplasma Mycoplasma hyopneumoniae Genes Bacterial Agarose gel electrophoresis Mollicutes Humans bacteria Serotyping Mycoplasma mycoides |
| Zdroj: | Nucleic Acids Research. 18:1451-1455 |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/18.6.1451 |
| Popis: | Contour clamped homogeneous field (CHEF) agarose gel electrophoresis (AGE), ramped to give linear separation of DNA molecules of 600-1600 kilobase pairs (kbp), was used to determine mobilities for full-sized genomic DNA of the serotype standard strains of the human genital mollicutes, Ureaplasma urealyticum relative to yeast chromosomal DNA markers. Indicated genome sizes (in kbp) were 760 for the four biotype 1 strains and 840-1140 for eleven biotype 2 strains. Other estimates were: 720 for Mycoplasma hominis, 1070 for Mycoplasma hyopneumoniae, 890 for Mycoplasma flocculare, 1180 and 1350 for Mycoplasma mycoides subsp. mycoides Y and GC1176-2, respectively, and 1650 and 1580 for Acholeplasma laidlawii B and PG 8, respectively. These data supplement previous evidence from CHEF AGE that the genomes of the Mycoplasmataceae are diverse in size with some larger than previously estimated from DNA renaturation kinetics. |
| Databáze: | OpenAIRE |
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