Immunoreactivity of sinusoids in hepatoblastoma: an immunohistochemical study using lectin UEA-1 and antibodies against endothelium-associated antigens, including CD34
| Autor: | J.-C. Xiao, Peter Ruck, Edwin Kaiserling |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Hepatoblastoma
CD31 Pathology medicine.medical_specialty Histology Endothelium CD34 Antigens Differentiation Myelomonocytic Antigens CD34 Biology Pathology and Forensic Medicine Sinusoid Antigens CD Lectins von Willebrand Factor Biomarkers Tumor medicine Humans Microcirculation Liver Neoplasms General Medicine medicine.disease digestive system diseases Staining Platelet Endothelial Cell Adhesion Molecule-1 medicine.anatomical_structure Liver Immunohistochemistry Endothelium Vascular Plant Lectins Cell Adhesion Molecules Immunostaining |
| Zdroj: | Histopathology. 26:451-455 |
| ISSN: | 0309-0167 |
| DOI: | 10.1111/j.1365-2559.1995.tb00253.x |
| Popis: | Sinusoids are found not only in the normal liver but also in certain liver tumours, including hepatoblastoma, the most common malignant liver tumour in childhood. In this study, sinusoids in 12 hepatoblastomas, of various subtypes, and in normal liver were investigated with UEA-1 and antibodies against von Willebrand's factor, CD31 and CD34 to detect differences of possible diagnostic significance. In the normal liver, staining of sinusoids was seen with all these markers, but it was focal and confined to a few sinusoids near the portal tracts. In hepatoblastoma, the endothelial markers reacted with the sinusoids to varying extents. UEA-1 and anti-CD34 usually stained the majority of these vessels, anti-CD34 staining greater numbers of sinusoids and with greater intensity. Immunostaining revealed that both number and spatial organization of sinusoids in hepatoblastoma are dependent on the subtype. In addition to staining of endothelium, one of the two small cell hepatoblastomas exhibited strong immunoreactivity of the tumour cells for CD34. These findings show that the marked difference in sinusoidal immunoreactivity for CD34 between normal liver and hepatoblastoma could be useful for discriminating between non-neoplastic liver tissue and highly differentiated fetal hepatoblastoma. Our findings also show that small cell hepatoblastoma, in addition to acute leukaemia, should be considered when immunoreactivity for CD34 is found in small round and blue cell tumours in childhood. |
| Databáze: | OpenAIRE |
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