Distinguishing nuclei-specific benzo[a]pyrene-induced effects from whole-cell alterations in MCF-7 cells using Fourier-transform infrared spectroscopy
| Autor: | Francis Martin, Blessing Obinaju, Nigel J. Fullwood |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
DNA damage
Breast Neoplasms Toxicology Resting Phase Cell Cycle S Phase chemistry.chemical_compound Spectroscopy Fourier Transform Infrared Toxicity Tests Benzo(a)pyrene Humans Fourier transform infrared spectroscopy Spectroscopy Cell Nucleus Biochemistry chemistry Attenuated total reflection Multivariate Analysis MCF-7 Cells Microscopy Electron Scanning Biophysics Pyrene Female Biomarkers Intracellular DNA DNA Damage |
| Zdroj: | Toxicology. 335:27-34 |
| ISSN: | 0300-483X |
| DOI: | 10.1016/j.tox.2015.07.001 |
| Popis: | Exposure to chemicals such as benzo[a]pyrene (B[a]P) can generate intracellular toxic mechanisms. Fourier-transform infrared (FTIR) spectroscopy is a novel approach that allows the non-destructive analysis of underlying chemical bond alterations in patho-physiological processes. This study set out to examine whether B[a]P-induced whole cell alterations could be distinguished from effects on nuclei of exposed cells. Using attenuated total reflection FTIR (ATR-FTIR) spectroscopy, alterations in nuclei isolated from B[a]P-treated MCF-7 cells concentrated either in G0/G1- or S-phase were observed. B[a]P-induced effects in whole-cells included alterations to lipids, DNA and protein spectral regions. Absorbance areas for protein and DNA/RNA regions in B[a]P-treated whole cells differed significantly (P |
| Databáze: | OpenAIRE |
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