Analysis of serum proteome profiles of non-Hodgkin lymphoma for biomarker identification
| Autor: | Xiao Rui Fu, Xing Ai Wu, Zhen Chang Sun, Wang Ma, Fei Fei Nan, Qing Xia Fan, Li Geng, Mingzhi Zhang, Rui Lin Wang |
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| Rok vydání: | 2008 |
| Předmět: |
Adult
Proteomics medicine.medical_specialty Adolescent Proteome medicine.medical_treatment Biophysics Biochemistry Gastroenterology Serology immune system diseases hemic and lymphatic diseases Internal medicine medicine Humans Aged Chemotherapy L-Lactate Dehydrogenase Chemistry Gene Expression Profiling Lymphoma Non-Hodgkin Computational Biology Blood Proteins Middle Aged medicine.disease Prognosis Blood proteins Lymphoma Gene expression profiling Gene Expression Regulation Neoplastic Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Immunology Biomarker (medicine) Differential diagnosis Biomarkers |
| Zdroj: | Journal of proteomics. 72(6) |
| ISSN: | 1876-7737 |
| Popis: | Serum samples from non-Hodgkin lymphoma (NHL) patients who had not undergone chemotherapy, lymphnoditis patients, and healthy adults were analyzed using surface-enhanced laser desorption-ionization time-of-flight mass spectrometry (SELDI-TOF MS) to detect the differentially expressed serum proteins. Models were developed to distinguish between the healthy adult group and the NHL group, with a sensitivity of 69% and specificity of 90%, and between the lymphnoditis group and the NHL group with a sensitivity of 74% and specificity of 84%. A protein with the m/z of M10 197.91 u was expressed at a significantly higher level in the NHL group, compared to the other groups. Furthermore, differences were also significant among different stages of NHL and among samples with different International Prognosis Index (IPI) scores or lactase dehydrogenase (LDH) levels. The three identified proteins may offer a new serological approach for early diagnosis, differential diagnosis, and pathogenic investigation of NHL. And the protein with the m/z of M10 197.91 u may be a new serological biomarker for monitoring treatment response and evaluating the prognosis of patients with NHL. |
| Databáze: | OpenAIRE |
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