Experimental autoimmune glomerulonephritis induced by homologous and isologous glomerular basement membrane in Brown-Norway rats
| Autor: | C. M. Lockwood, Charles D. Pusey, S. J. Cashman, D. J. Evans, Martin J. Holland, R. A. Sinico, J.-J. Lloveras |
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| Přispěvatelé: | Pusey, C, Holland, M, Cashman, S, Sinico, R, Lloveras, J, Evans, D, Lockwood, C |
| Jazyk: | angličtina |
| Rok vydání: | 1991 |
| Předmět: |
Male
medicine.medical_specialty Isoantigens Glomerular deposits Interstitial nephritis Kidney Glomerulus urologic and male genital diseases Focal Glomerulonephritis Autoantigens Autoimmune Disease Immunoglobulin G Basement Membrane Autoimmune Diseases Glomerulonephritis Autoantigen Internal medicine Rats Inbred BN Medicine Animals Glomerulonephriti Autoantibodies Isoantigen Transplantation biology urogenital system business.industry Animal Glomerular basement membrane Autoantibody Rats Inbred Strains medicine.disease Autoantibodie female genital diseases and pregnancy complications Rats Disease Models Animal medicine.anatomical_structure Endocrinology Nephrology biology.protein Rat business Kidney Glomerulu Nephritis |
| Popis: | In order to study disease mechanisms and potential forms of therapy in glomerulonephritis, a model of experimental autoimmune glomerulonephritis (EAG) has been developed in the rat. We have examined the response of Brown-Norway (BN) rats to a single i.m. injection of collagenase-solubilised homologous (Sprague-Dawley, SD) or isologous (BN) glomerular basement membrane (GBM), with and without complete Freund's adjuvant (CFA). There was a dose-dependent circulating anti-GBM antibody response to all preparations of rat GBM. Animals given either antigen alone at a dose of 2 mg/kg developed circulating anti-GBM antibodies, which reached peak values by 6 weeks (63 +/- 5% following SD GBM; 53 +/- 8% following BN GBM), but did not develop glomerular deposits of IgG or nephritis. Animals given 2 mg/kg SD GBM in CFA developed greater concentrations of anti-GBM antibody by 6 weeks (122 +/- 20%) together with linear deposits of IgG on glomerular and tubular basement membranes (TBM), albuminuria (mean 7 mg/24 h), and variable focal segmental necrotising glomerulonephritis with mild interstitial nephritis. The same dose of BN GBM in CFA produced similar concentrations of circulating antibody (144 +/- 26%), with linear deposits of IgG on GBM but rarely TBM, little albuminuria, and variable mild focal glomerulonephritis. Other strains injected with SD GBM in CFA showed a variable circulating anti-GBM antibody response, which was similar to that of BN rats in PVG and DA rats but lower in LEW and WAG rats. Linear deposits of IgG on the GBM were detected in a proportion of PVG and DA rats, but not in LEW or WAG rats.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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