Identification and expression analysis of microRNAs in medaka gonads
| Autor: | Yefei Zhu, Mingyou Li, Kerang Chen, Yun Wu, Weiwei Qiu, Cancan Yuan |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Small RNA Gonad Oryzias Sexism Ovary 03 medical and health sciences Oogenesis microRNA Genetics medicine Animals Gonads Spermatogenesis Gene In Situ Hybridization Fluorescence Gene Library medicine.diagnostic_test biology Gene Expression Profiling Gene Expression Regulation Developmental General Medicine biology.organism_classification Cell biology MicroRNAs 030104 developmental biology medicine.anatomical_structure Organ Specificity Female Development of the gonads Fluorescence in situ hybridization |
| Zdroj: | Gene. 646:210-216 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/j.gene.2017.12.062 |
| Popis: | Gonad development is a highly regulated, coordinated biological process and increasing evidences have indicated that microRNA (miRNA) may be involved in this dynamic program. Medaka (Oryzias latipes) is a good model for reproductive research as it has distinct sex determining genes, however, research in gonadal miRNAs is lacked. In this study, two small RNA libraries from the ovaries and testes were constructed and sequenced. A total of 285 conserved and 388 novel miRNAs were obtained, among which 142 mature miRNAs were significantly (> two-fold change) up or down regulated in the testis compared to the ovary. Quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) analysis showed that miR-430c, miR-26a and miR-202-5p were expressed in a gonad-specific or sex-biased pattern. Fluorescence in situ hybridization (FISH) indicated that miR-202-5p was present throughout spermatogenesis and was only detected at the early stages of oogenesis, this sex biased expression pattern suggested that miR-202-5p might be a crucial candidate in male differentiation and development. Our study provides the repertoire, a comprehensive annotation of miRNAs from gonads and a reference for functional studies of miRNAs in medaka. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect