Visualization of exosomes from mesenchymal stem cells in vivo by magnetic resonance imaging
| Autor: | Xinhua Wei, Tianqi Liu, Xuegang Xin, Yurong Zhu, Ruiting Zhao |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
Recombinant Fusion Proteins Biomedical Engineering Biophysics Bone Marrow Cells Exosomes 030218 nuclear medicine & medical imaging Mice 03 medical and health sciences 0302 clinical medicine Genes Reporter In vivo Image Processing Computer-Assisted Animals Humans Radiology Nuclear Medicine and imaging Mesenchymal stem cell proliferation Lactadherin Chemistry Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Milk Proteins Magnetic Resonance Imaging Fusion protein In vitro Microvesicles Cell biology Mice Inbred C57BL Phenotype Antigens Surface Ferritins Stem cell Oxidoreductases 030217 neurology & neurosurgery |
| Zdroj: | Magnetic Resonance Imaging. 68:75-82 |
| ISSN: | 0730-725X |
| Popis: | Background and purpose We develop a method of imaging exosomes in vivo according to the vital role of exosomes in intercellular communication. This study aims to design a new label method that allows the visualization of labeled exosomes with magnetic resonance imaging (MRI). Methods We designed a fusion protein consisting of two parts, namely, ferritin heavy chain (FTH1) and a truncated lactadherin. FTH1 is used as an MRI reporter. Lactadherin is a trans-membrane protein. The lactadherin protein are mostly located on the outer surface of exosomes. We replaced the outer membrane part of lactadherin with FTH1, infected mesenchymal stem cells with lentivirus carrying the fusion protein, and isolated exosomes from the labeled cells by ultracentrifugation. Labeled exosomes were validated by transmission electron microscopy images, Western blot, nanosight particle tracking, and visualized in vitro and in vivo by MRI. Results FTH1 expression would suppress mesenchymal stem cell proliferation, whereas the characterization of labeled exosomes remains comparable with unlabeled exosomes. MR imaging shows that exosomes labeled with FTH1 can be visualized in vitro and in vivo. Conclusion This innovative reporter–imaging approach to track and visualize exosomes with MRI can be utilized as a tool for the study of the role of exosomes under different conditions. |
| Databáze: | OpenAIRE |
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