The release of osteoclast-stimulating factors on supraphysiological loading by osteoprogenitors coincides with expression of genes associated with inflammation and cytoskeletal arrangement
| Autor: | Bratengeier, Cornelia, Bakker, Astrid D., Liszka, Aneta, Schilcher, Jörg, Fahlgren, Anna |
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| Přispěvatelé: | Oral Cell Biology, Elderly care medicine |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Inflammation
Cancer och onkologi Multidisciplinary Stem Cells Cell- och molekylärbiologi Kirurgi RANK Ligand Osteoclasts Deformation dynamics Extracellular signalling molecules Differentiation Targeted bone remodelling Cell Differentiation Osteogenesis Cancer and Oncology Humans Surgery Cell and Molecular Biology |
| Zdroj: | Scientific Reports, 12:21578, 1-15. Nature Publishing Group Scientific Reports, 12(1):21578. Nature Publishing Group Bratengeier, C, Bakker, A D, Liszka, A, Schilcher, J & Fahlgren, A 2022, ' The release of osteoclast-stimulating factors on supraphysiological loading by osteoprogenitors coincides with expression of genes associated with inflammation and cytoskeletal arrangement ', Scientific Reports, vol. 12, 21578, pp. 1-15 . https://doi.org/10.1038/s41598-022-25567-7 |
| ISSN: | 2045-2322 |
| Popis: | Supraphysiological loading induced by unstable orthopedic implants initiates osteoclast formation, which results in bone degradation. We aimed to investigate which mechanosensitive cells in the peri-implant environment produce osteoclast-stimulating factors and how the production of these factors is stimulated by supraphysiological loading. The release of osteoclast-stimulating factors by different types of isolated bone marrow-derived hematopoietic and mesenchymal stem cells from six osteoarthritic patients was analyzed after one hour of supraphysiological loading (3.0 ± 0.2 Pa, 1 Hz) by adding their conditioned medium to osteoclast precursors. Monocytes produced factors that enhanced osteoclastogenesis by 1.6 ± 0.07-fold and mesenchymal stem cells by 1.4 ± 0.07-fold. Medium from osteoprogenitors and pre-osteoblasts enhanced osteoclastogenesis by 1.3 ± 0.09-fold and 1.4 ± 0.03-fold, respectively, where medium from four patients elicited a response and two did not. Next generation sequencing analysis of osteoprogenitors revealed that genes encoding for inflammation-related pathways and cytoskeletal rearrangements were regulated differently between responders and non-responders. Our data suggest that released osteoclast-stimulating soluble factors by progenitor cells in the bone marrow after supraphysiological loading may be related to cytoskeletal arrangement in an inflammatory environment. This connection could be relevant to better understand the aseptic loosening process of orthopedic implants. Funding: Knut and Alice Wallenberg Foundation; Wallenberg Centre for Molecular Medicine (WCMM), Linkoping University, Linkoping, Sweden; Region ostergotland; Swedish Research Council [2016-01822, 2019-03636]; ALF [Ro-941258, Ro-969530] |
| Databáze: | OpenAIRE |
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