Overexpressed Rce1 is positively correlated with tumor progression and predicts poor prognosis in prostate cancer

Autor: Li Mai, Meicai Li, Jianjun Li, Ying Li, Qiangfeng Zeng, Jie Hu, Jiang He, Wenqiang Wu, Delin Wang, Liangliang Huang, Maolin Xiao, Wujiang Liu, Yunfeng He
Rok vydání: 2015
Předmět:
0301 basic medicine
Male
Pathology
medicine.medical_specialty
Time Factors
Blotting
Western
Prostatic Hyperplasia
Kaplan-Meier Estimate
urologic and male genital diseases
Real-Time Polymerase Chain Reaction
Pathology and Forensic Medicine
03 medical and health sciences
Prostate cancer
0302 clinical medicine
Prostate
Risk Factors
Endopeptidases
medicine
Biomarkers
Tumor
Humans
RNA
Messenger
Neoplasm Metastasis
Aged
Neoplasm Staging
Proportional Hazards Models
Chi-Square Distribution
Proportional hazards model
business.industry
Transurethral Resection of Prostate
Prostatic Neoplasms
Hyperplasia
Middle Aged
medicine.disease
Immunohistochemistry
Up-Regulation
030104 developmental biology
Real-time polymerase chain reaction
medicine.anatomical_structure
Treatment Outcome
Tumor progression
030220 oncology & carcinogenesis
Case-Control Studies
Multivariate Analysis
Cancer research
Disease Progression
Biomarker (medicine)
Neoplasm Grading
business
Zdroj: Human pathology. 47(1)
ISSN: 1532-8392
Popis: Ras and a-factor-converting enzyme 1 (Rce1) have been reported to play a key role in the proteolysis processing of Ras proteins. The present study investigated the prognostic significance of Rce1 in patients with prostate cancer (PCa). The expressions of the mRNA and protein of Rce1 were analyzed in 12 pairs of PCa and benign prostatic hyperplasia (BPH) by quantitative real-time polymerase chain reaction and Western blotting, respectively. Immunohistochemistry was used to examine expression of Rce1 protein in 74 PCa tissues and 30 BPH tissues. The association between Rce1 expression and the specific clinicopathologic features was evaluated by χ(2) tests. Kaplan-Meier and Cox proportional hazards regression models were used to analyze the data. We found that expression of Rce1 mRNA and protein was markedly higher in PCa tissues than in paired BPH tissues. Expression of Rce1 in PCa was strongly associated with clinicopathologic features. It was detected in 69 (93.24%) of 74 PCa tissues by immunohistochemistry, and it was found to be associated with Gleason score (P = .013), T class (P = .015), and distant metastasis (P = .044). Patients with PCa having higher Rce1 expression had substantially shorter survival times than patients with lower Rce1 expression. Univariate and multivariate analysis revealed that Rce1 was an independent prognostic factor. In conclusion, our study suggests that expression of Rce1 can serve as an independent biomarker for the prognosis of PCa patients.
Databáze: OpenAIRE
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