Destabilization of ROS metabolism in tomato roots as a phytotoxic effect of meta-tyrosine
| Autor: | Joanna Olechowicz, Katarzyna Ciacka, Olga Andrzejczak, Agnieszka Gniazdowska, Paweł Staszek, Renata Bogatek, Kim H. Hebelstrup, Urszula Krasuska |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Membrane permeability Physiology Plant Science medicine.disease_cause 01 natural sciences Plant Roots Non-protein amino acid Superoxide dismutase Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound Solanum lycopersicum Genetics medicine Tyrosine Plant Proteins chemistry.chemical_classification Reactive oxygen species biology Catalase Phenolic compounds Protein carbonylation 030104 developmental biology chemistry Biochemistry Oxidative stress biology.protein Antioxidant enzymes Lipid Peroxidation Reactive Oxygen Species 010606 plant biology & botany Phenylalanine analog |
| Zdroj: | Andrzejczak, O, Krasuska, U, Olechowicz, J, Staszek, P, Ciacka, K, Bogatek, R, Hebelstrup, K & Gniazdowska, A 2018, ' Destabilization of ROS metabolism in tomato roots as a phytotoxic effect of meta-tyrosine ', Plant Physiology and Biochemistry, vol. 123, pp. 369-377 . https://doi.org/10.1016/j.plaphy.2017.12.024 |
| ISSN: | 1873-2690 |
| DOI: | 10.1016/j.plaphy.2017.12.024 |
| Popis: | meta -Tyrosine ( m -Tyr) is a non-protein amino acid produced in both plants and animals. Primary mode of action of this phenylalanine analog is its incorporation into protein structure leading to formation of aberrant molecules. An increased level of m -Tyr in animal cells is detected under oxidative stress and during age-related processes characterized by overproduction of reactive oxygen species (ROS). The aim of this study was to link m -Tyr physiological action to disturbances in ROS metabolism in tomato ( Solanum lycopersicum L.) seedlings roots. Treatment of tomato seedlings with m -Tyr (50 or 250 μM) for 24–72 h led to inhibition of root growth without a lethal effect. Toxicity of m -Tyr after 72 h was connected with an increase in hydrogen peroxide concentration in roots and ROS leakage into the surrounding medium. On the contrary, membrane permeability and lipid peroxidation in roots were the same as for the control. This was accompanied by a decrease in total antioxidant activity and an increased accumulation of phenolic compounds. Catalase (CAT) activity declined in roots exposed to 50 μM m -Tyr after 24 h while after 72 h activity of this enzyme was inhibited in both treated and non-treated samples. Activities of different superoxide dismutase (SOD) isoforms were similar in m -Tyr stressed roots and in the control. Prolonged culture resulted in decrease of transcript level of genes coding CAT and SOD with the exception of FeSOD. Moreover, m -Tyr increased the level of protein carbonyl groups indicating induction of oxidative stress as a non-direct mode of action. |
| Databáze: | OpenAIRE |
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