Replication functions of pC194 are necessary for efficient plasmid transduction by M13 phage
| Autor: | I Jones, M Dagert, Stanislav Dusko Ehrlich, S Romac, B. Niaudet, A. Goze |
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| Rok vydání: | 1984 |
| Předmět: |
DNA Replication
Genes Viral viruses Phagemid Biology Virus Replication medicine.disease_cause Coliphages General Biochemistry Genetics and Molecular Biology Viral Proteins chemistry.chemical_compound Plasmid Transduction Genetic Escherichia coli medicine Amino Acid Sequence Molecular Biology T-DNA Binary system Base Sequence General Immunology and Microbiology General Neuroscience DNA replication DNA Restriction Enzymes Molecular biology PBR322 Genes Viral replication chemistry bacteria Staphylococcus Phages DNA Research Article Plasmids |
| Zdroj: | The EMBO Journal. 3:81-86 |
| ISSN: | 0261-4189 |
| DOI: | 10.1002/j.1460-2075.1984.tb01764.x |
| Popis: | Escherichia coli plasmids pBR313 and pBR322 were transduced by phage M13 with low efficiency (10(-8) transductants/phage). Hybrid plasmids pHV12 or pHV33, composed of Staphylococcus aureus plasmid pC194 and pBR313 or pBR322, respectively, were transduced much more efficiently (10(-4) transductants/phage). Inactivation of either of the two zones necessary for pC194 replication, one coding for a protein, the other not, reduced the transforming efficiency of hybrids to the level of pBR322. Activity of the pC194 replication region was not necessary for the formation of chimeras between M13 and the transduced plasmid in the donor cells, but rather for the establishment of the plasmid in the recipient cells. |
| Databáze: | OpenAIRE |
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