Cardiac myosin heavy chain expression during heart development in Xenopus laevis
| Autor: | Anton W. Neff, William G. Cox |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Cancer Research
DNA Complementary Transcription Genetic Molecular Sequence Data Xenopus Myosins Biology MyoD Polymerase Chain Reaction Xenopus laevis Myosin Gene expression medicine Animals Amino Acid Sequence Molecular Biology In Situ Hybridization Base Sequence Sequence Homology Amino Acid Heart development Gene Expression Regulation Developmental Heart Cell Biology Blotting Northern biology.organism_classification Molecular biology Somite medicine.anatomical_structure Neurula Organ Specificity MYF5 Developmental Biology |
| Zdroj: | Differentiation. 58:269-280 |
| ISSN: | 0301-4681 |
| Popis: | Muscle-specific gene expression in the heart during Xenopus development was investigated using reverse transcription-polymerase chain reaction (RT-PCR) and whole-mount in situ hybridization to detect transcripts of the gene for the cardiac myosin heavy chain (CMHC). RT-PCR analysis determined that CMHC transcripts are present in the cardiac mesoderm at state 13, demonstrating that muscle-specific gene expression in the primitive myocardium has begun by the early neurula stage, approximately 30 h before the heart beat begins. Xenopus, therefore, is similar to amniotes and mammals in that cardiac precursor cells begin to express muscle-specific gene transcripts soon after commitment to the cardiac myocyte lineage. The earliest CMHC gene transcripts can be detected in the heart using whole-mount in situ hybridization is early tailbud stage 28, which coincides with the onset of heart tube morphogenesis. CMHC gene expression was also detected in skeletal muscle: RT-PCR analysis determined that CMHC transcripts are transiently expressed in the somite during the initial phases of skeletal muscle differentiation. Furthermore, CMHC mRNAs are expressed in a subset of head muscles of the feeding tadpole. CMHC gene expression is induced in ectodermal cells of the animal cap in blastula-stage embryos injected with synthetic MyoD or Myf5 RNA, suggesting that the CMHC gene contains regulatory elements that are responsive to the activity of those skeletal-muscle-specific transcription factors. |
| Databáze: | OpenAIRE |
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