The fatty acid elongase Bond is essential for Drosophila sex pheromone synthesis and male fertility
| Autor: | Kah Junn Tan, Jacqueline S. R. Chin, Wan Chin Ng, Joanne Y. Yew |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Fatty Acid Elongases General Physics and Astronomy Gas Chromatography-Mass Spectrometry Mass Spectrometry Article General Biochemistry Genetics and Molecular Biology Animals Genetically Modified Acetyltransferases Botany Animals Drosophila Proteins RNA Messenger Sex Attractants Gene Chromatography High Pressure Liquid Genetics Multidisciplinary biology Gene Expression Profiling General Chemistry Mating Preference Animal biology.organism_classification Gene expression profiling Drosophila melanogaster Fertility Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Sex pheromone Pheromone Drosophila Drosophila simulans Female Ectopic expression Transcription Initiation Site Drosophila Protein |
| Zdroj: | Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Insects use a spectacular variety of chemical signals to guide their social behaviours. How such chemical diversity arises is a long-standing problem in evolutionary biology. Here we describe the contribution of the fatty acid elongase Bond to both pheromone diversity and male fertility in Drosophila. Genetic manipulation and mass spectrometry analysis reveal that the loss of bond eliminates the male sex pheromone (3R,11Z,19Z)-3-acetoxy-11,19-octacosadien-1-ol (CH503). Unexpectedly, silencing bond expression severely suppresses male fertility and the fertility of conspecific rivals. These deficits are rescued on ectopic expression of bond in the male reproductive system. A comparative analysis across six Drosophila species shows that the gain of a novel transcription initiation site is correlated with bond expression in the ejaculatory bulb, a primary site of male pheromone production. Taken together, these results indicate that modification of cis-regulatory elements and subsequent changes in gene expression pattern is one mechanism by which pheromone diversity arises. Insect behaviours are often guided by chemical signals, but little is known about how pheromone diversity evolves. Here the authors show that loss of the gene bond in Drosophila eliminates the sex pheromone CH503, while silencing it reduces the fertility of males and their conspecific rivals. |
| Databáze: | OpenAIRE |
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