Evaluation of S-RBD and high specificity ACE-2-binding antibodies on SARS-CoV-2 patients after six months from infection
| Autor: | Francesca Gisone, Serena Sarubbi, Massimo Pieri, Francesco Spinazzola, Cristina Ricotta, Monica Borgatti, Flaminia Tomassetti, Marco Ciotti, Sergio Bernardini, Monica Cagnoli, Marco Iannetta, Marzia Nuccetelli, Massimo Andreoni, Graziella Calugi |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Adult
Male Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Immunology Antibodies Viral Neutralizing antibodies Asymptomatic Article Settore MED/06 Serology NO Neutralizing antibodies Vaccines Serological tests SARS-CoV-2 S-RBD antibodies Immunity Humans Immunology and Allergy Medicine Serologic Tests Longitudinal Studies Receptor Aged Aged 80 and over Pharmacology chemistry.chemical_classification Vaccines biology business.industry Serological tests SARS-CoV-2 COVID-19 Middle Aged Antibodies Neutralizing Enzyme S-RBD antibodies chemistry Angiotensin-converting enzyme 2 biology.protein Female Angiotensin-Converting Enzyme 2 medicine.symptom Antibody business |
| Zdroj: | International Immunopharmacology |
| Popis: | The antibody response to SARS-CoV-2 has not yet fully defined, but the availability of sensitive and specific serological assays is crucial to observe the presence of specific antibodies against the human receptor binding domain (S-RBD) and high specificity ACE-2-binding antibodies or neutralizing antibodies (NT) in response to vaccines. Indeed, these peculiar antibodies should prevent viral interaction between RBD and Angiotensin-Converting Enzyme 2 (ACE2) receptor, located on surface of host cells. In this study, 72 samples from 37 hospitalized COVID-19 patients and 35 not-hospitalized patients were analyzed longitudinally. The detection of S-RBD and NT antibodies was carried out using CLIA tests. Hospitalized patients showed elevated serum levels of S-RBD (97.22%) and NT (77.78%) antibodies, differently, not-hospitalized, who were paucisymptomatic or asymptomatic patients, showed lower serum levels of S-RBD (65.71%) and NT (38.14%) antibodies. The results suggest that the NT serum level is strongly related to disease severity (p |
| Databáze: | OpenAIRE |
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