Characterization of natural hybrids of Saccharomyces cerevisiae and Saccharomyces bayanus var. uvarum
| Autor: | Michel Aigle, Christine Le Jeune, Marc Lollier, Claude Erny, Isabelle Masneuf-Pomarède, Catherine Demuyter, Jean Luc Legras |
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| Přispěvatelé: | Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA)), Laboratoire Vigne, Biotechnologies et Environnement, Laboratoire Vigne Biotechnologie et Environnement (LVBE), Santé de la vigne et qualité du vin (SVQV), Institut National de la Recherche Agronomique (INRA)-Université Louis Pasteur - Strasbourg I, Université Claude Bernard Lyon 1 (UCBL), Université de Lyon, Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro) |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
SACCHAROMYCES VAR. UVARUM
Saccharomyces cerevisiae Saccharomyces bayanus Wine Polymerase Chain Reaction Applied Microbiology and Biotechnology Microbiology Saccharomyces 03 medical and health sciences DNA Fungal Mycological Typing Techniques 030304 developmental biology Genetics Gel electrophoresis SACCHAROMYCES CEREVISIAE 0303 health sciences biology 030306 microbiology General Medicine biology.organism_classification Yeast [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Karyotyping Fermentation Hybridization Genetic Microsatellite HYBRID CELLS France Ploidy Restriction fragment length polymorphism Microsatellite Repeats |
| Zdroj: | FEMS Yeast Research FEMS Yeast Research, Oxford University Press (OUP), 2007, 7 (4), pp.540-549. ⟨10.1111/j.1567-1364.2007.00207.x⟩ |
| ISSN: | 1567-1356 1567-1364 |
| Popis: | International audience; Nine yeast strains were isolated from spontaneous fermentations in the Alsace area of France, during the 1997, 1998 and 1999 grape harvests. Strains were characterized by pulsed-field gel electrophoresis, PCR–restriction fragment length polymorphism (RFLP) of the MET2 gene, d-PCR, and microsatellite patterns. Karyotypes and MET2 fragments of the nine strains corresponded to mixed chromosomal bands and restriction patterns for both Saccharomyces cerevisiae and Saccharomyces bayanus var. uvarum. They also responded positively to amplification with microsatellite primers specific to both species and were demonstrated to be diploid. However, meiosis led to absolute nonviability of their spores on complete medium. All the results demonstrated that the nine yeast strains isolated were S. cerevisiae_S. bayanus var. uvarum diploid hybrids. Moreover, microsatellite DNA analysis identified strains isolated in the same cellar as potential parents belonging to S. bayanus var. uvarum and S. cerevisiae. |
| Databáze: | OpenAIRE |
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