Contact of myeloma cells induces a characteristic transcriptome signature in skeletal precursor cells -Implications for myeloma bone disease
Autor: | Regina Ebert, Alexander Keller, Franz Jakob, Robert J. Tower, Doris Schneider, Katrin Schlegelmilch, S. Zeck, Norbert Schütze, Julia Dotterweich, Beate Geyer |
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Rok vydání: | 2015 |
Předmět: |
0301 basic medicine
Male Stromal cell Histology Physiology Cellular differentiation Endocrinology Diabetes and Metabolism Antigens CD19 Osteogenic precursor cells Cell Communication Biology Plasma cell Bone and Bones Transcriptome 03 medical and health sciences Osteogenesis Precursor cell Bone disease ddc:570 Cell Line Tumor medicine Cell Adhesion Angiopoietin-Like Protein 4 Humans Angiopoietin-like 4 ddc:610 Multiple myeloma Aged B-Lymphocytes Gene Expression Profiling Mesenchymal stem cell Reproducibility of Results Cell Differentiation Mesenchymal Stem Cells Middle Aged medicine.disease Molecular biology Coculture Techniques Cell biology Up-Regulation Gene Expression Regulation Neoplastic 030104 developmental biology medicine.anatomical_structure Phenotype Female Bone Diseases Multiple Myeloma Monoclonal gammopathy of undetermined significance |
Zdroj: | Bone. 93 |
ISSN: | 1873-2763 |
Popis: | Physical interaction of skeletal precursors with multiple myeloma cells has been shown to suppress their osteogenic potential while favoring their tumor-promoting features. Although several transcriptome analyses of myeloma patient-derived mesenchymal stem cells have displayed differences compared to their healthy counterparts, these analyses insufficiently reflect the signatures mediated by tumor cell contact, vary due to different methodologies, and lack results in lineage-committed precursors.To determine tumor cell contact-mediated changes on skeletal precursors, we performed transcriptome analyses of mesenchymal stem cells and osteogenic precursor cells cultured in contact with the myeloma cell line INA-6. Comparative analyses confirmed dysregulation of genes which code for known disease-relevant factors and additionally revealed upregulation of genes that are associated with plasma cell homing, adhesion, osteoclastogenesis, and angiogenesis. Osteoclast-derived coupling factors, a dysregulated adipogenic potential, and an imbalance in favor of anti-anabolic factors may play a role in the hampered osteoblast differentiation potential of mesenchymal stem cells. Angiopoietin-Like 4 (ANGPTL4) was selected from a list of differentially expressed genes as a myeloma cell contact-dependent target in skeletal precursor cells which warranted further functional analyses. Adhesion assays with full-length ANGPTL4-coated plates revealed a potential role of this protein in INA-6 cell attachment.This study expands knowledge of the myeloma cell contact-induced signature in the stromal compartment of myelomatous bones and thus offers potential targets that may allow detection and treatment of myeloma bone disease at an early stage. |
Databáze: | OpenAIRE |
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