Pre-validation of a MALDI MS proteomics-based method for the reliable detection of blood and blood provenance
Autor: | Malcolm R. Clench, Simona Francese, Katie Kennedy, Cameron Heaton, Vaughn G. Sears, Glenn Langenburg, Laura M. Cole, Mark Sealey, Tom Clark, Richard McColm |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
Male
Proteomics Maldi ms lcsh:Medicine 01 natural sciences Article 03 medical and health sciences 0302 clinical medicine Species Specificity Semen Tandem Mass Spectrometry Animals Humans Medicine False Positive Reactions 030216 legal & forensic medicine lcsh:Science Animal species Pre validation Multidisciplinary Chromatography Staining and Labeling business.industry Biological techniques lcsh:R 010401 analytical chemistry Ms analysis Gold standard (test) Forensic Medicine Body Fluids 0104 chemical sciences Blood Stains Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization lcsh:Q Crime business Biomarkers Blood Chemical Analysis Chromatography Liquid |
Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-18 (2020) Scientific Reports |
ISSN: | 2045-2322 |
Popis: | The reliable identification of blood, as well as the determination of its origin (human or animal) is of great importance in a forensic investigation. Whilst presumptive tests are rapid and deployed in situ, their very nature requires confirmatory tests to be performed remotely. However, only serological tests can determine blood provenance. The present study improves on a previously devised Matrix Assisted Laser Desorption Ionisation Mass Spectrometry (MALDI MS)—proteomics based method for the reliable detection of blood by enabling the determination of blood provenance. The overall protocol was developed to be more specific than presumptive tests and faster/easier than the gold standard liquid chromatography (LC) MS/MS analysis. This is considered a pre-validation study that has investigated stains and fingermarks made in blood, other biofluids and substances that can elicit a false-positive response to colorimetric or presumptive tests, in a blind fashion. Stains and marks were either untreated or enhanced with a range of presumptive tests. Human and animal blood were correctly discriminated from other biofluids and non-biofluid related matrices; animal species determination was also possible within the system investigated. The procedure is compatible with the prior application of presumptive tests. The refined strategy resulting from iterative improvements through a trial and error study of 56 samples was applied to a final set of 13 blind samples. This final study yielded 12/13 correct identifications with the 13th sample being correctly identified as animal blood but with no species attribution. This body of work will contribute towards the validation of MALDI MS based methods and deployment in violent crimes involving bloodshed. |
Databáze: | OpenAIRE |
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