Genetic and Molecular Characterization of a Cryptochrome from the Filamentous Fungus Neurospora crassa
| Autor: | William J. Belden, Martha Merrow, Till Roenneberg, Chen-Hui Chen, Jay C. Dunlap, Cornelia Madeti, Jennifer J. Loros, Allan C. Froehlich |
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| Přispěvatelé: | Beersma lab |
| Rok vydání: | 2010 |
| Předmět: |
Time Factors
Light White Collar-1 Circadian clock Mutant chemistry.chemical_compound Cryptochrome Gene Expression Regulation Fungal Amino Acids DNA Fungal DNA PHOTOLYASE Conserved Sequence Flavin adenine dinucleotide Fungal protein biology Articles General Medicine ARABIDOPSIS Circadian Rhythm Cell biology DROSOPHILA Phenotype MAMMALIAN CIRCADIAN CLOCK Flavin-Adenine Dinucleotide Protein Binding EXPRESSION endocrine system Molecular Sequence Data BLUE-LIGHT PHOTORECEPTORS HIGH-THROUGHPUT Microbiology Neurospora Neurospora crassa Fungal Proteins WHITE COLLAR-1 Folic Acid Biological Clocks FEEDBACK LOOPS Escherichia coli Amino Acid Sequence RNA Messenger Molecular Biology Binding Sites PHOTOLYASE ACTIVITY RNA Fungal biology.organism_classification Molecular biology Cryptochromes chemistry Pyrimidine Dimers Mutation |
| Zdroj: | Eukaryotic Cell, 9(5), 738-750 |
| ISSN: | 1535-9786 1535-9778 |
| Popis: | In plants and animals, cryptochromes function as either photoreceptors or circadian clock components. We have examined the cryptochrome from the filamentous fungus Neurospora crassa and demonstrate that Neurospora cry encodes a DASH-type cryptochrome that appears capable of binding flavin adenine dinucleotide (FAD) and methenyltetrahydrofolate (MTHF). The cry transcript and CRY protein levels are strongly induced by blue light in a wc-1 -dependent manner, and cry transcript is circadianly regulated, with a peak abundance opposite in phase to frq . Neither deletion nor overexpression of cry appears to perturb the free-running circadian clock. However, cry disruption knockout mutants show a small phase delay under circadian entrainment. Using electrophoretic mobility shift assays (EMSA), we show that CRY is capable of binding single- and double-stranded DNA (ssDNA and dsDNA, respectively) and ssRNA and dsRNA. Whole-genome microarray experiments failed to identify substantive transcriptional regulatory activity of cry under our laboratory conditions. |
| Databáze: | OpenAIRE |
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