Genetic and Molecular Characterization of a Cryptochrome from the Filamentous Fungus Neurospora crassa

Autor: William J. Belden, Martha Merrow, Till Roenneberg, Chen-Hui Chen, Jay C. Dunlap, Cornelia Madeti, Jennifer J. Loros, Allan C. Froehlich
Přispěvatelé: Beersma lab
Rok vydání: 2010
Předmět:
Time Factors
Light
White Collar-1
Circadian clock
Mutant
chemistry.chemical_compound
Cryptochrome
Gene Expression Regulation
Fungal

Amino Acids
DNA
Fungal

DNA PHOTOLYASE
Conserved Sequence
Flavin adenine dinucleotide
Fungal protein
biology
Articles
General Medicine
ARABIDOPSIS
Circadian Rhythm
Cell biology
DROSOPHILA
Phenotype
MAMMALIAN CIRCADIAN CLOCK
Flavin-Adenine Dinucleotide
Protein Binding
EXPRESSION
endocrine system
Molecular Sequence Data
BLUE-LIGHT PHOTORECEPTORS
HIGH-THROUGHPUT
Microbiology
Neurospora
Neurospora crassa
Fungal Proteins
WHITE COLLAR-1
Folic Acid
Biological Clocks
FEEDBACK LOOPS
Escherichia coli
Amino Acid Sequence
RNA
Messenger

Molecular Biology
Binding Sites
PHOTOLYASE ACTIVITY
RNA
Fungal

biology.organism_classification
Molecular biology
Cryptochromes
chemistry
Pyrimidine Dimers
Mutation
Zdroj: Eukaryotic Cell, 9(5), 738-750
ISSN: 1535-9786
1535-9778
Popis: In plants and animals, cryptochromes function as either photoreceptors or circadian clock components. We have examined the cryptochrome from the filamentous fungus Neurospora crassa and demonstrate that Neurospora cry encodes a DASH-type cryptochrome that appears capable of binding flavin adenine dinucleotide (FAD) and methenyltetrahydrofolate (MTHF). The cry transcript and CRY protein levels are strongly induced by blue light in a wc-1 -dependent manner, and cry transcript is circadianly regulated, with a peak abundance opposite in phase to frq . Neither deletion nor overexpression of cry appears to perturb the free-running circadian clock. However, cry disruption knockout mutants show a small phase delay under circadian entrainment. Using electrophoretic mobility shift assays (EMSA), we show that CRY is capable of binding single- and double-stranded DNA (ssDNA and dsDNA, respectively) and ssRNA and dsRNA. Whole-genome microarray experiments failed to identify substantive transcriptional regulatory activity of cry under our laboratory conditions.
Databáze: OpenAIRE