Molecular diagnosis of lymphoblastic leukemia
| Autor: | S.V.S. Prasad, Seetha Dayakar, Kalal Iravathy Goud, Amina Shaik, Koteshwar N Rao, S Vanjakshi |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Myeloid Karyotype Chromosomal translocation Acute lymphoblastic leukemia Biology lcsh:RC254-282 Translocation Genetic Bone Marrow hemic and lymphatic diseases medicine Humans Radiology Nuclear Medicine and imaging In Situ Hybridization Fluorescence Gene Rearrangement Acute leukemia medicine.diagnostic_test Breakpoint Infant General Medicine Gene rearrangement Histone-Lysine N-Methyltransferase karyotyping Precursor Cell Lymphoblastic Leukemia-Lymphoma lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens medicine.disease Molecular biology fluoroscence in situ hybridization medicine.anatomical_structure Oncology flowcytometry Myeloid-Lymphoid Leukemia Protein Fluorescence in situ hybridization Lymphoid leukemia |
| Zdroj: | Journal of Cancer Research and Therapeutics, Vol 9, Iss 3, Pp 493-496 (2013) |
| ISSN: | 1998-4138 |
| Popis: | The mixed lineage leukemia (MLL) gene at chromosome band 11q23 is commonly involved in reciprocal translocations that is detected in acute leukemia. The MLL gene, coomonly known as mixed lineage leukemia or myeloid lymphoid leukemia, has been independently identified and cloned from the 11q23 breakpoint of acute leukemia. We describe a patient with acute lymphoblastic leukemia whose cells had shown reciprocal translocation between short arm (p21) of chromosome 2 and long arm (q23) of chromosome number 11 [t(2;11) (p21;q23)] by cytogenetic analysis. Fluorescence in situ hybridization analysis (FISH) was also performed for reconfirmation with a probe for MLL which showed split signals, hybridizing to both the derivative 2 and 11 chromosomes. Our study confirmed FISH as the most suitable assay for detecting MLL rearrangements because of its sensitivity and speed. It recommended that FISH should be used as complementary to conventional cytogenetic analysis. In conclusion, evaluation of the t(2;11)(p21;q23) was done by molecular clarification and flow cytometry. |
| Databáze: | OpenAIRE |
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