Rotavirus particles in the extrahepatic bile duct in experimental biliary atresia
| Autor: | Anika Dreier, Claus Petersen, Christina Oetzmann von Sochaczewski, Jean-Pierre Timmermans, J. F. Kuebler, Inge Brouns, Isabel Pintelon, Christian Klemann |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Rotavirus
Pathology medicine.medical_specialty viruses medicine.medical_treatment Biology Liver transplantation medicine.disease_cause Rotavirus Infections Virus Mice Microscopy Electron Transmission Bile Ducts Extrahepatic Biliary Atresia Biliary atresia medicine Animals Cellular localization Tropism Mice Inbred BALB C Microscopy Confocal Reverse Transcriptase Polymerase Chain Reaction Bile duct virus diseases General Medicine Viral Load medicine.disease Disease Models Animal medicine.anatomical_structure Atresia Pediatrics Perinatology and Child Health RNA Viral Surgery Human medicine |
| Zdroj: | Journal of pediatric surgery |
| ISSN: | 0022-3468 |
| DOI: | 10.1016/j.jpedsurg.2013.09.064 |
| Popis: | Background Biliary atresia (BA) is the most common indication for liver transplantation in children. The experimental model of BA, induced by rotavirus infection in neonatal mice, has been widely used to investigate the inflammatory aspects of this disease. We investigated the kinetics and the localization of the viral infection in this murine model. Methods In this study 399 animals were employed for a detailed investigation of rhesus rotavirus (RRV)-induced BA. RRV kinetics was analyzed by rtPCR and its (sub) cellular localization investigated using whole mounts which were further processed for confocal and electron microscopy. Results The BA mouse model resulted in up to 100% induction of atresia following RRV injection. The kinetics of RRV infection differed between liver and extrahepatic bile ducts. While the virus peak up to day 10 postinfection was similar in both organs, the virus remained detectable in extrahepatic bile duct cells up to day 21. Interestingly, RRV particles were localized not only in cholangiocytes but also in cells of the subepithelial layers, potentially macrophages. Conclusions RRV remains present in the extrahepatic bile duct cells after an initial virus peak. Viral particles were detected in subepithelial cells in contrast to the described tropism toward cholangiocytes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect