A comparison of the effect of molluscum contagiosum virus MC159 and MC160 proteins on vaccinia virus virulence in intranasal and intradermal infection routes
| Autor: | Sunetra Biswas, Edward J. Roy, Geoffrey L. Smith, Joanna L. Shisler, Brian M. Ward |
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| Přispěvatelé: | Smith, Geoffrey [0000-0002-3730-9955], Apollo - University of Cambridge Repository |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Injections Intradermal Short Communication viruses 030106 microbiology Virulence Context (language use) Vaccinia virus Virus 03 medical and health sciences chemistry.chemical_compound Tissue culture Viral Proteins Immune system Virology intradermal infection Animals Humans MC159 Child Administration Intranasal Mice Inbred BALB C Molluscum contagiosum virus biology pathogenesis MC160 NF-kappa B biology.organism_classification 030104 developmental biology Viral replication chemistry poxvirus Female Vaccinia circulatory and respiratory physiology |
| Popis: | Molluscum contagiosum virus (MCV) causes persistent, benign skin neoplasm in children and adults. MCV is refractive to growth in standard tissue culture and there is no relevant animal model of infection. Here we investigated whether another poxvirus (vaccinia virus; VACV) could be used to examine MCV immunoevasion protein properties in vivo. The MCV MC159L or MC160L genes, which encode NF-κB antagonists, were inserted into an attenuated VACV lacking an NF-κB antagonist (vΔA49), creating vMC159 and vMC160. vMC160 slightly increased vΔA49 virulence in the intranasal and intradermal routes of inoculation. vMC159 infection was less virulent than vΔA49 in both inoculation routes. vMC159-infected ear pinnae did not form lesions, but virus replication still occurred. Thus, the lack of lesions was not due to abortive virus replication. This system provides a new approach to examine MCV immunoevasion proteins within the context of a complete and complex immune system. |
| Databáze: | OpenAIRE |
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