The effects of the 11 kDa protein and the putative X protein on the p6 promoter activity of Parvovirus B19 in Hela cells
| Autor: | Jianxin Peng, Peng Xu, Yu Huang, Yanming Dong, Ying Wang, Yongbo Yang, Peter Tijssen, Kaiyu Liu, Yi Li |
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| Přispěvatelé: | College of Life Sciences, Central China Normal University [Wuhan, China], Institut Armand Frappier (INRS-IAF), Institut National de la Recherche Scientifique [Québec] (INRS)-Réseau International des Instituts Pasteur (RIIP), Bioengineering Department, Wuhan Bioengineering Institute, This work was funded by the National Natural Science Foundation of China (30670081). |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Gene Expression Regulation
Viral MESH: Parvovirus B19 Human viruses [SDV]Life Sciences [q-bio] Viral Nonstructural Proteins CREB Virus chemistry.chemical_compound MESH: Gene Expression Regulation Viral Virology HSPA2 MESH: Promoter Regions Genetic Parvovirus B19 Human Genetics Humans Binding site Promoter Regions Genetic Molecular Biology Regulation of gene expression MESH: Humans biology Parvovirus Epithelial Cells Promoter General Medicine biology.organism_classification Molecular biology chemistry MESH: Epithelial Cells MESH: HeLa Cells biology.protein MESH: Viral Nonstructural Proteins DNA HeLa Cells |
| Zdroj: | Virus Genes Virus Genes, Springer Verlag, 2013, 46 (1), pp.167-9. ⟨10.1007/s11262-012-0839-1⟩ |
| ISSN: | 0920-8569 1572-994X |
| DOI: | 10.1007/s11262-012-0839-1⟩ |
| Popis: | International audience; Human parvovirus B19 (B19) is a small nonenveloped icosahedral virus with a single-stranded, linear 5.6 kb DNA genome. The p6 promoter, at map unit 6 of the viral genome, controls the expression of all B19 transcripts. Some previous reports revealed that this promoter is transactivated by NS1 protein. In an attempt to investigate the roles of other small viral proteins in the control of the p6 promoter activity, various truncated promoter/reporter constructs along with these nonstructural protein expression vectors were introduced into Hela cells. The results showed that the putative X protein upregulated the activity of p6 promoter significantly, but that the 11 kDa protein did not. Furthermore, the possible responsive DNA elements for X protein were identified to be located primarily between nt 265 and 343 of the p6 promoter region. In addition, we observed that deletion of the potential ATF/CREB binding sites located in 5' terminal nucleotide influenced the activity of p6 promoter significantly. |
| Databáze: | OpenAIRE |
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