High Performance Liquid Chromatography Tandem Mass Spectrometry Measurement of Bimatoprost, Latanoprost and Travoprost in Eyelash Enhancing Cosmetic Serums
| Autor: | Simona Pichini, Maria Concetta Rotolo, Emilia Marchei, Silvia Graziano, Carmine Guarino, Daniela De Orsi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Aging
prostaglandins analogs high performance liquid chromatography Pharmaceutical Science Dermatology Tandem mass spectrometry travoprost 01 natural sciences High-performance liquid chromatography lcsh:Chemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Chemical Engineering (miscellaneous) Latanoprost Chromatography Bimatoprost 010401 analytical chemistry Selected reaction monitoring Reversed-phase chromatography 0104 chemical sciences bimatoprost latanoprost eyelash enhancers Prostaglandin analog chemistry lcsh:QD1-999 030221 ophthalmology & optometry Surgery Travoprost medicine.drug |
| Zdroj: | Cosmetics, Vol 3, Iss 1, p 4 (2016) Cosmetics; Volume 3; Issue 1; Pages: 4 |
| ISSN: | 2079-9284 |
| Popis: | Most common prostaglandin analogs, bimatoprost, latanoprost and travoprost, are licensed for the reduction of elevated intraocular pressure in patients with open angle glaucoma and ocular hypertension, but their non approved use as eyelash enhancers is becoming popular, especially in patients with eyelashes hypotrichosis. A fast and sensitive high performance liquid chromatography tandem mass spectrometry method was developed for the measurement of bimatoprost, latanoprost and travoprost in cosmetic serums freely web-sold to increase eyelash length, thickness and darkness. The analytes and the internal standard (reserpine) were separated by reversed phase chromatography with 5 mM ammonium acetate with 0.02% formic acid (mobile phase A) and 5 mM ammonium acetate in acetonitrile/water (95/5; v/v) with 0.02% formic acid (mobile phase B) by gradient elution and detected with tandem mass spectrometry operated in multiple reaction monitoring mode. Linearity between 1 and 500 μg/g shows good correlation coefficients (r2 = 0.99) for all substances. Analytical recovery of analytes under investigation were always higher than 90% and intra-assay and inter-assay precision and accuracy always better than 11%. This method was successfully applied to analyze cosmetic serums freely sold on the Internet websites. |
| Databáze: | OpenAIRE |
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