Isolation of segmented filamentous bacteria from complex gut microbiota
| Autor: | Giedre Turner, Stacey M Meeker, Charlie C Hsu, Aaron C. Ericsson, Lillian Maggio-Price, Craig L. Franklin, Lisa Montoya, Annie E. Wolfe |
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| Rok vydání: | 2015 |
| Předmět: |
Bacteriological Techniques
Gram-negative bacteria biology Segmented filamentous bacteria Gastrointestinal Microbiome Candidatus Arthromitus Gut flora biology.organism_classification Isolation (microbiology) General Biochemistry Genetics and Molecular Biology Microbiology Disease Models Animal Mice Immune system Gram-Negative Bacteria Animals Bacteria Biotechnology |
| Zdroj: | BioTechniques. 59:94-98 |
| ISSN: | 1940-9818 0736-6205 |
| DOI: | 10.2144/000114319 |
| Popis: | Segmented filamentous bacteria (SFB) modulate the ontogeny of the immune system, and their presence can significantly affect mouse models of disease. Until recently, the inability to successfully culture SFB has made controlled studies on the mechanisms by which these bacteria exert their influence problematic. Here, we report a new method for selecting SFB from complex microbial mixtures, providing researchers a simple and cost-effective means to prepare pure infective inocula for prospective studies and also to compare individual SFB isolates. |
| Databáze: | OpenAIRE |
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