All-optical mapping of barrel cortex circuits based on simultaneous voltage-sensitive dye imaging and channelrhodopsin-mediated photostimulation
| Autor: | Judy Sng, George J. Augustine, Shun Qiang Lo, Dawn X. P. Koh |
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| Rok vydání: | 2015 |
| Předmět: |
Materials science
Radiological and Ultrasound Technology Special Section Papers Neuroscience (miscellaneous) Voltage-sensitive dye Channelrhodopsin Optogenetics Barrel cortex Brain mapping Photostimulation Premovement neuronal activity Radiology Nuclear Medicine and imaging Voltage-Sensitive Dye Imaging Neuroscience |
| Zdroj: | Neurophotonics. 2:021013 |
| ISSN: | 2329-423X |
| Popis: | We describe an experimental approach that uses light to both control and detect neuronal activity in mouse barrel cortex slices: blue light patterned by a digital micromirror array system allowed us to photostimulate specific layers and columns, while a red-shifted voltage-sensitive dye was used to map out large-scale circuit activity. We demonstrate that such all-optical mapping can interrogate various circuits in somatosensory cortex by sequentially activating different layers and columns. Further, mapping in slices from whisker-deprived mice demonstrated that chronic sensory deprivation did not significantly alter feedforward inhibition driven by layer 5 pyramidal neurons. Further development of voltage-sensitive optical probes should allow this all-optical mapping approach to become an important and high-throughput tool for mapping circuit interactions in the brain. |
| Databáze: | OpenAIRE |
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