Macro-to-micro porous special bioactive glass and ceftriaxone-sulbactam composite drug delivery system for treatment of chronic osteomyelitis: an investigation through in vitro and in vivo animal trial
| Autor: | Debabrata Basu, Biswanath Kundu, Sudip Dasgupta, Aruna Kumari Singh, Prasenjit Mukherjee, Rupnarayan Bhattacharya, Someswar Datta, Partha Das, Subhasis Roy, Tapan Kumar Mandal, Samit Kumar Nandi |
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| Rok vydání: | 2010 |
| Předmět: |
Staphylococcus aureus
Materials science medicine.drug_class Antibiotics Biomedical Engineering Biophysics Bioengineering Bone and Bones law.invention Biomaterials Drug Delivery Systems law In vivo medicine Animals Drug Carriers Osteomyelitis Ceftriaxone Biomaterial Sulbactam Hydrogen-Ion Concentration medicine.disease Anti-Bacterial Agents Bioactive glass Drug delivery Chronic Disease Adsorption Glass Rabbits Powders Drug carrier Porosity medicine.drug Biomedical engineering |
| Zdroj: | Journal of materials science. Materials in medicine. 22(3) |
| ISSN: | 1573-4838 |
| Popis: | A systematic and extensive approach incorporating in vitro and in vivo experimentation to treat chronic osteomyelitis in animal model were made using antibiotic loaded special bioactive glass porous scaffolds. After thorough characterization for porosity, distribution, surface charge, a novel drug composite were infiltrated by using vacuum infiltration and freeze-drying method which was subsequently analyzed by SEM–EDAX and studied for in vitro drug elution in PBS and SBF. Osteomyelitis in rabbit was induced by inoculation of Staphylococcus aureus and optimum drug-scaffold were checked for its efficacy over control and parenteral treated animals in terms of histopathology, radiology, in vivo drug concentration in bone and serum and implant-bone interface by SEM. It was optimized that 60P samples with 60–65% porosity (bimodal distribution of macro- to micropore) with average pore size ~60 μm and higher interconnectivity, moderately high antibiotic adsorption efficiency (~49%) was ideal. Results after 42 days showed antibiotic released higher than MIC against S. aureus compared to parenteral treatment (2 injections a day for 6 weeks). In vivo drug pharmacokinetics and SEM on bone-defect interface proved superiority of CFS loaded porous bioactive glass implants over parenteral group based on infection eradication and new bone formation. |
| Databáze: | OpenAIRE |
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