False positive serology of prepandemic chagasic samples with SARS‐CoV ‐2 antigen

Autor:	Juan D H, Zuluaga, Juan C, Santos-Barbosa, Adriana, Cuellar, Concepción J, Puerta, John M, Gonzalez
Rok vydání:	2022
Předmět:	Infectious Diseases SARS-CoV-2 Spike Glycoprotein Coronavirus Public Health Environmental and Occupational Health Humans COVID-19 Chagas Disease Parasitology Antibodies Viral Sensitivity and Specificity
Zdroj:	Tropical Medicine & International Health. 27:1009-1012
ISSN:	1365-3156 1360-2276
DOI:	10.1111/tmi.13818
Popis:	To determine whether prepandemic sera from patients with Chagas disease recognise SARS-CoV-2 antigens.Forty sera from patients with Chagas disease were tested for the presence of IgG cross-reactivity against the nucleocapsid protein (NP) and spike (S) SARS-CoV-2 proteins by ELISA. Positive samples were tested again using a different ELISA and CLIA, both against NP.None of the sera from patients with Chagas disease, previously confirmed as positive for the presence of anti-Trypanosoma cruzi antibodies reacted against the SARS-CoV-2 S protein, and six samples tested positive for the NP antigen (15%). The six positive samples were re-tested, five remained positive by ELISA and all were negative by CLIA.According to our data, false-positive results might be a concern in the detection of SARS-CoV-2 antibodies in patients with Chagas disease.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c2c2f6fe8f09ac0b299df7be3ec7850e https://doi.org/10.1111/tmi.13818 Zobrazit plný text záznamu Plný text