High COX-2 expression in cancer-associated fibiroblasts contributes to poor survival and promotes migration and invasiveness in nasopharyngeal carcinoma

Autor: Chunmei Kuang, Chen Shi, Yinghong Zhu, Liang Zeng, Songqing Fan, Shilian Chen, Jian Ouyang, Weihong Jiang, Xin Zhang, Jiaojiao Guo, Wen Zhou, Xuan Wu, Jiliang Xia, Guizhu Liu, Yongjun Guan, Xingxing Jian, Yangbowen Wu
Rok vydání: 2019
Předmět:
Zdroj: Molecular Carcinogenesis
ISSN: 1098-2744
Popis: Nasopharyngeal carcinoma (NPC) has the highest rate of metastasis among head and neck cancers, and distant metastasis is the major reason for treatment failure. We have previously shown that high cyclooxygenase‐2 (COX‐2) expression is associated with a poor prognosis of patients with NPC and inhibits chemotherapy‐induced senescence in NPC cells. In this study, we found that COX‐2 was upregulated in cancer‐associated fibroblasts (CAFs) derived from NPC by RNA‐Seq. Furthermore, elevated COX‐2 expression in CAF was detected in NPC patients with poor survival and distant metastasis by using immunohistochemistry. Then, we identified that COX‐2 is highly expressed in CAF at the distant metastasis site in seven paired NPC patients. High expression of COX‐2 and secretion of prostaglandin E2, a major product catalyzed by COX‐2 in fibroblasts, promotes migration and invasiveness of NPC cells in vitro. On the contrary, inhibition of COX‐2 has the opposite effect in vitro as well as in the COX‐2−/− mouse with the lung metastasis model in vivo. Mechanistically, we discovered that COX‐2 elevates tumor necrosis factor‐α expression in CAF to promote NPC cell migration and invasiveness. Overall, our results identified a novel target in CAF promoting NPC metastasis. Our findings suggested that high expression of COX‐2 in CAF may serve as a new prognostic indicator for NPC metastasis and provide the possibility of targeting CAF for treating advanced NPC.
Databáze: OpenAIRE
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