Factors affecting the performance of P22 ELISA for the diagnosis of caprine tuberculosis in milk samples
| Autor: | J, Ortega, J A, Infantes-Lorenzo, A, Roy, L, de Juan, B, Romero, I, Moreno, M, Domínguez, L, Domínguez, J, Bezos |
|---|---|
| Přispěvatelé: | Ministerio de Ciencia e Innovación (España), Ministerio de Agricultura, Pesca y Alimentación (España), Ministerio de Ciencia, Innovación y Universidades (España) |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | E-Prints Complutense. Archivo Institucional de la UCM instname Repisalud Instituto de Salud Carlos III (ISCIII) |
| Popis: | Caprine tuberculosis (TB) is a zoonosis caused by members of the Mycobacterium tuberculosis complex (MTBC). Caprine TB eradication programmes are based mainly on intradermal tuberculin tests and slaughterhouse surveillance. However, the use of serological test has been extended as a potential diagnostic tool in goats through the use of serum, plasma, or even milk samples. Milk production and the antibodies (Ab) present in milk can vary depending on several circumstances. In the present study, different factors that may affect the performance of humoral TB diagnosis were analysed using goat milk samples: 1) lactation stage, 2) a recent previous skin test (booster effect) and 3) the effect of freeze-thaw cycles on milk samples preserved with azidiol. TB-infected animals (n = 44) were selected to evaluate the evolution of the Ab levels during the 6-month lactation period, along with its potential effect on the P22 ELISA results. In general, no significant changes (p = 0.079) were observed throughout the study as regards Ab levels in milk samples between consecutive analysis although the reactivity to P22 ELISA decreased when samplings were performed at the last two months of the lactation. Regarding the booster effect, the quantitative results showed a significant variation (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|