Bovine fetal DNA in the maternal circulation: Applications and implications
| Autor: | Ester Silveira Ramos, Daniela Cristina Lemos, Álvaro Fabrício Lopes Rios, A. Araújo, P. L. Takeuchi, H.C. Lemos |
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| Rok vydání: | 2011 |
| Předmět: |
Male
medicine.medical_specialty Placenta Mothers Gestational Age Sexing Biology Andrology Fetus Sex Factors Bovines Pregnancy Internal medicine Prenatal Diagnosis Obstetrics and Gynaecology medicine Animals Maternal-Fetal Exchange Embryos Obstetrics and Gynecology Gestational age Transplacental Embryo TSPY gene DNA medicine.disease Endocrinology medicine.anatomical_structure Reproductive Medicine Gestation Pregnancy Animal Cattle Female Developmental Biology |
| Zdroj: | Placenta. 32(11) |
| ISSN: | 1532-3102 |
| Popis: | Objective The main aim of the present study was to detect bovine fetal DNA in the maternal circulation, a relatively unexplored subject in the literature. Study design DNA was extracted from blood of 84 primipara cows ( Bos indicus ) at different gestational ages (30–270 days) and from 100 adult animals (50 males and 50 non-pregnant cows). The samples were analyzed using PCR with primers for TSPY gene. Results Molecular results matched the fetal phenotypic gender in all 47 male and 37 female fetuses, including early pregnancy, and in control animals. Conclusions These results evidence a bovine transplacental fetal DNA passage. |
| Databáze: | OpenAIRE |
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