Human Gingival Lymphocytes. I. Methodology for the Isolation of Human Gingival Lymphocytes
Autor: | D. L. Woodson, J.A. Withers, P.A. O'Neill, A. Herrin, Elbert Coker, L. Friedman, Bruce F. Mackler |
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Rok vydání: | 1979 |
Předmět: |
Adult
0301 basic medicine Adolescent Plasma Cells Gingiva Cell Separation Microbiology 03 medical and health sciences 0302 clinical medicine Digestion (alchemy) medicine Cell separation Humans Lymphocytes General Dentistry Periodontal Diseases Chemistry 030206 dentistry Middle Aged Isolation (microbiology) Microbial Collagenase 030104 developmental biology Microbial collagenase Immunology Collagenase Collagen medicine.drug |
Zdroj: | Journal of Dental Research. 58:1946-1952 |
ISSN: | 1544-0591 0022-0345 |
DOI: | 10.1177/00220345790580100101 |
Popis: | Various methodologies were examined for the isolation of inflammatory cells from diseased human gingiva. The best recovery of viable gingival lymphocytes (gMNC) was achieved by a method which combined initial collagenase digestion followed by gentle teasing with an 18-gauge needle. |
Databáze: | OpenAIRE |
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