Malondialdehyde-Modified HDL Leads to Accumulation of Cholesterol in Rat Liver Endothelial Cells
| Autor: | Emile Levy, B. Tuchweber, V. Gavino, F. Guertin, S. Brunet |
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| Rok vydání: | 1995 |
| Předmět: |
Male
medicine.medical_specialty Endothelium Kupffer Cells Biophysics Biochemistry Bile Acids and Salts Rats Sprague-Dawley chemistry.chemical_compound High-density lipoprotein In vivo Malondialdehyde Internal medicine medicine Animals Bile Molecular Biology Cholesterol Reverse cholesterol transport nutritional and metabolic diseases Biological Transport Cell Biology Rats Endothelial stem cell Kinetics medicine.anatomical_structure Endocrinology Liver chemistry lipids (amino acids peptides and proteins) Lipoproteins HDL Lipoprotein |
| Zdroj: | Biochemical and Biophysical Research Communications. 212:1-8 |
| ISSN: | 0006-291X |
| Popis: | In order to study in vivo the effect of modified high density lipoprotein (HDL) on the movement of free cholesterol to liver cells and bile, we injected i.v. into rats, native or malondialdehyde modified HDL labelled with [14C]cholesterol. Bile analysis indicated that the contribution of labelled cholesterol to bile acid secretion was diminished in the group receiving MDA-modified HDL when compared to control group. On the other hand, the liver analysis revealed higher radioactivity in the treated group. A separation of liver cells into parenchymal, endothelial, and kupffer at 90 min after the injection of MDA-modified HDL or native HDL indicated that the endothelial cell uptake of labelled free cholesterol from MDA-modified HDL was 2.6-fold higher than for native HDL. It is suggested that liver endothelial cells may be involved in the protection against atherogenoic oxidized lipoprotein. However, with regard to our finding, the uptake of cholesterol from modified HDL was detrimental to bile acid secretion. |
| Databáze: | OpenAIRE |
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