Development of a near-infrared fluorescence ELISA method using tyramide signal amplification
| Autor: | D. Michael Olive, Lael Cheung, Mark Cradduck, Haibiao Gong |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Infrared Rays
Biophysics Biotin Tyramine Enzyme-Linked Immunosorbent Assay Near infrared fluorescence Biochemistry Horseradish peroxidase Substrate Specificity Elisa kit Animals Humans Elisa method Molecular Biology Horseradish Peroxidase biology Epidermal Growth Factor Chemistry Human epidermal growth factor Substrate (chemistry) Cell Biology Molecular biology Fluorescence Immunoglobulin G biology.protein Signal amplification |
| Zdroj: | Analytical biochemistry. 426(1) |
| ISSN: | 1096-0309 |
| Popis: | In this study, we applied tyramide signal amplification (TSA) to fluorescence enzyme-linked immunosorbent assay (ELISA) employing horseradish peroxidase (HRP) as the detection enzyme. When used with a human epidermal growth factor ELISA kit, the TSA method led to a >100-fold increase in fluorescence signal intensity in comparison to an unamplified method. It also showed wider dynamic range and better sensitivity compared to a conventional method using tetramethylbenzidine as the HRP substrate. |
| Databáze: | OpenAIRE |
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