Encapsulation and self-superparasitism of Pseudapanteles dignus (Muesebeck) (Hymenoptera: Braconidae), a parasitoid of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)
| Autor: | Marcela Inés Schneider, Nicolas Desneux, María Gabriela Luna |
|---|---|
| Přispěvatelé: | Centro de Estudios Parasitologicos y de Vectores [La Plata] (CEPAVE), Universidad Nacional de la Plata [Argentine] (UNLP)-Comisión de Investigaciones Científicas [Buenos Aires] (CIC)-Consejo Nacional de Investigaciones Científicas y Técnicas [Buenos Aires] (CONICET), Institut Sophia Agrobiotech (ISA), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA), ANPCYT (AR) [IM-40-2000, PICT 2012-1624], CONICET [PIP 0112, PIP 0205], Universidad Nacional de La Plata [SA N 706], Marie Curie Actions-International Research Staff Exchange Scheme, IRSES (IPRABIO), Erjun Ling, Centro de Estudios Parasitológicos y de Vectores (CEPAVE ), Institut Sophia Agrobiotech [Sophia Antipolis] (ISA), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (... - 2019) (UNS), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), Luna, Maria G. |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
Life Cycles Hemocytes Physiology Oviposition [SDV]Life Sciences [q-bio] Cultivos Agrícolas lcsh:Medicine biological control Pathogenesis Hymenoptera Pathology and Laboratory Medicine 01 natural sciences Parasitoid purl.org/becyt/ford/1 [https] White Blood Cells Larvae Animal Cells Reproductive Physiology Medicine and Health Sciences Parasitología lcsh:Science Microscopy Multidisciplinary Ecology biology Light Microscopy Agriculture Plants Trophic Interactions Insects Lepidoptera Community Ecology endoparasitoid Moths and Butterflies Parasitism Larva Host-Pathogen Interactions Cellular Types Braconidae CIENCIAS NATURALES Y EXACTAS Research Article animal structures Arthropoda Immune Cells Otras Ciencias Biológicas Immunology Zoology Crops Research and Analysis Methods 010603 evolutionary biology Fruits Host-Parasite Interactions Lepidoptera genitalia Ciencias Biológicas Tomatoes Botany Animals Ciencias Naturales Pest Control Biological purl.org/becyt/ford/1.6 [https] Life Cycle Stages Blood Cells Ecology and Environmental Sciences lcsh:R fungi Organisms Biology and Life Sciences Cell Biology biology.organism_classification Gelechiidae Invertebrates Species Interactions 010602 entomology hemocite Microscopy Electron Scanning Instar Tuta absoluta lcsh:Q immunological defense Developmental Biology Crop Science |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2016, 11 (10), pp.1-10. ⟨10.1371/journal.pone.0163196⟩ CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET PLoS ONE, Vol 11, Iss 10, p e0163196 (2016) SEDICI (UNLP) Universidad Nacional de La Plata instacron:UNLP Plos One 10 (11), 1-10. (2016) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0163196⟩ |
| Popis: | Endoparasitoids can be killed by host encapsulation, a cellular-mediated host immunological response against parasitism that involves hemocytes aggregation. As a counteracting strategy, many parasitoids can evade this host response through self-superparasitism. The objectives of this study were: 1) to describe the parasitoid Pseudapanteles dignus (Hymenoptera: Braconidae) early immature stages (egg and larva) encapsulation by the host Tuta absoluta (Lepidoptera: Gelechiidae), and 2) to determine the occurrence of self-superparasitism and the rate of escaping to encapsulation of this parasitoid. Knowledge of host-parasitoid immunological interaction is crucial when evaluating the potential of an endoparasitoid as a biological control candidate. Parasitoid-exposed T. absoluta larvae were dissected in vivo under light stereoscope microscope at 24-h intervals, for five days after exposition to detect encapsulation. The preimaginal stages of P. dignus and numbers of healthy and encapsulated immature parasitoids per host were recorded. Samples of parasitoid eggs and larvae were processed for SEM visualization of encapsulation. Necropsies evidenced that only the early first larval instar of P. dignus (up to 96 h-old) was partially or completely encapsulated. A non-melanized capsule, formed by layers of granulocyte-type hemocytes enveloping around the parasitoid body, was recorded. Approximately 50% of the parasitized T. absoluta larvae had significantly only one P. dignus egg, meanwhile supernumerary parasitization yielded up to seven immature parasitoids per host. The proportion of single-early first larval instar of P. dignus reached ô 0.5 and decreased significantly as the number of parasitoid individuals per host increased. P. dignus encapsulation and its ability to overcome with the host immune defense through self-superparasitism indicate that T. absoluta is a semi-permissive host for this parasitoid. Centro de Estudios Parasitológicos y de Vectores |
| Databáze: | OpenAIRE |
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