Sulfonamide resistance gene for plant transformation
| Autor: | Colin Robinson, Philip M. Mullineaux, Louise Brooks, François Guerineau, A. Lucy, Julie W. Meadows |
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| Rok vydání: | 1990 |
| Předmět: |
Genetic Markers
Chloroplasts R Factors Molecular Sequence Data Plant Science Chimeric gene Biology Marker gene Transformation Genetic Tobacco Genetics Amino Acid Sequence Cloning Molecular Gene Selectable marker Dihydropteroate Synthase Sulfonamides Base Sequence fungi food and beverages General Medicine Plants Fusion protein Chloroplast stroma Transformation (genetics) Plants Toxic Biochemistry Dihydropteroate synthase Genetic Engineering Agronomy and Crop Science |
| Zdroj: | Plant molecular biology. 15(1) |
| ISSN: | 0167-4412 |
| Popis: | The sulfonamide resistance gene from plasmid R46 encodes for a mutated dihydropteroate synthase insensitive to inhibition by sulfonamides. Its coding sequence was fused to the pea ribulose bisphosphate carboxylase/oxygenase transit peptide sequence. Incubation of isolated chloroplasts with the fusion protein synthesised in vitro, showed that the bacterial enzyme was transported to the chloroplast stroma and processed into a mature form. Expression of the gene fusion in transgenic plants resulted in a high level of resistance to sulfonamides. Direct selection of transformed shoots on leaf explants was efficient using sulfonamides as sole selective agents. Transformed shoots rooted normally on sulfonamides at concentrations toxic for untransformed ones. Sulfonamide resistance was transmitted to the progeny of transformed plants as a single Mendelian dominant character. These results demonstrate that this chimeric gene can be used as an efficient and versatile selectable marker for plant transformation. |
| Databáze: | OpenAIRE |
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