An engineered oilseed crop produces oil enriched in two very long chain polyunsaturated fatty acids with potential health-promoting properties
| Autor: | Xiao Qiu, Robert G. Chapman, Yan Chen, Dauenpen Meesapyodsuk, Shengjian Ye, Yi Chen |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Crops Agricultural Linoleic acid Bioengineering Genetically modified crops Brassica 01 natural sciences Applied Microbiology and Biotechnology Metabolic engineering 03 medical and health sciences chemistry.chemical_compound Biosynthesis 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase Plant Oils Food science Plant Proteins chemistry.chemical_classification biology Chemistry Brassica carinata biology.organism_classification Plants Genetically Modified Yeast 030104 developmental biology Docosahexaenoic acid Fatty Acids Unsaturated Ranunculaceae 010606 plant biology & botany Biotechnology Polyunsaturated fatty acid |
| Zdroj: | Metabolic engineering. 49 |
| ISSN: | 1096-7184 |
| Popis: | Very long chain polyunsaturated fatty acids (VLCPUFAs) are well recognized for their health benefits in humans and animals. Here we report that identification and characterization of a gene (EhELO1) encoding the first functional ELO type elongase (3-ketoacyl-CoA synthase) in higher plants that is involved in the biosynthesis of two VLCPUFAs docosadienoic acid (DDA, 22:2n-6) and docosatrienoic acid (DTA, 22:3n-3) that possess potential health-promoting properties. Functional analysis of the gene in yeast indicated that this novel enzyme could elongate a wide range of polyunsaturated fatty acids with 18-22 carbons and effectively catalyze the biosynthesis of DDA and DTA by the sequential elongations of linoleic acid and alpha-linolenic acid, respectively. Seed-specific expression of this gene in oilseed crop Brassica carinata showed that the transgenic plants produced the level of DDA and DTA at approximately 30% of the total fatty acids in seeds, and the amount of the two fatty acids remained stable over four generations. The oilseed crop producing a high and sustained level of DDA and DTA provides an opportunity for high value agricultural products for nutritional and medical uses. |
| Databáze: | OpenAIRE |
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