Pollen grain development and male sterility in the perfect flowers of Maytenus obtusifolia Mart. (Celastraceae)

Autor: Lygia Dolores Ribeiro de Santiago-Fernandes, Bárbara de Sá-Haiad, Isabella Veríssimo Nader Haddad, Silvia Rodrigues Machado
Přispěvatelé: Universidade Federal do Rio de Janeiro (UFRJ), Universidade Estadual Paulista (Unesp)
Rok vydání: 2018
Předmět:
Zdroj: Web of Science
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
ISSN: 1615-6102
0033-183X
DOI: 10.1007/s00709-018-01336-0
Popis: Made available in DSpace on 2019-10-04T12:37:13Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-05-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Perfect flowers of Maytenus obtusifolia have partial sterility of pollen grains, resulting in collapsed and developed free microspores. However, the cellular events resulting in partial male sterility have not been determined. In pistillate flowers of this species, male sterility is related to the premature programmed cell death (PCD) in tapetum and sporogenic cells. The process occurs through autophagy via macroautophagy and massive autophagy and is associated with sporophytic cytoplasmic male sterility (CMS). Here, we characterised the development of pollen grains and investigated the cellular events that result in tapetum cells and free microspores PCD in perfect flowers, using light and transmission electron microscopy combined with the TUNEL (Terminal deoxynucleotidyl transferase mediated dUDP end-Labeling) assay and the ZIO (Zinc iodide-osmium tetroxide) method. Pollen grain development in perfect flowers was divided into eight developmental stages based on the characteristics of the pollen grains. Tapetum cells undergo PCD at the free microspore stage, through a macroautophagic process, by formation of autophagosomes and by autophagosomes giving rise to lytic vacuoles at maturity. In the final stage of PCD, massive autophagy occurs by rupture of the tonoplast. The development of viable and inviable microspores diverges at the vacuolated microspore stage, when PCD occurs in some free microspores, causing interruption of pollen development through necrosis. These events result in the observed partial male sterility. Viable microspores undergo mitosis and develop into tricellular pollen grains. Male sterility in hermaphrodite individuals is here interpreted as gametophytic CMS. Univ Fed Rio de Janeiro, Dept Bot, Museu Nacl, BR-22940040 Rio De Janeiro, RJ, Brazil Univ Estadual Paulista, Dept Bot, Inst Biociencias, BR-18618000 Botucatu, SP, Brazil Univ Estadual Paulista, Dept Bot, Inst Biociencias, BR-18618000 Botucatu, SP, Brazil FAPERJ: E-26/111.207/2014 CNPq: 447624/2014-8 CNPq: 304396/2015-0
Databáze: OpenAIRE
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