Effect of oxidative stress and calcium deregulation on FAM26F (CALHM6) expression during hepatitis B virus infection
| Autor: | Kehkshan Jabeen, Shaheen Shahzad, Uzma Malik, Sajid Mansoor, Saadia Zahid, Aneela Javed |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Adult Male Hepatitis B virus Adolescent Calcium deregulation Predictive marker Biology medicine.disease_cause lcsh:Infectious and parasitic diseases Cell Line 03 medical and health sciences Young Adult Immune system In vivo Immunity medicine Humans lcsh:RC109-216 Hepatitis B virus (HBV) Calcium Signaling Child Family with sequence similarity 26 member F (FAM26F) Innate immune system Membrane Glycoproteins 030102 biochemistry & molecular biology NAC virus diseases Reactive oxygen species (ROS) Hep G2 Cells Middle Aged Hepatitis B Calcium chelators Oxidative Stress 030104 developmental biology Infectious Diseases Gene Expression Regulation Cell culture Case-Control Studies Immunology Calcium Female IRF3 Research Article |
| Zdroj: | BMC Infectious Diseases BMC Infectious Diseases, Vol 21, Iss 1, Pp 1-13 (2021) |
| ISSN: | 1471-2334 |
| Popis: | Background Family with sequence similarity 26, member F (FAM26F) is an important innate immunity modulator playing a significant role in diverse immune responses, however, the association of FAM26F expression with HBV infection is not yet known. Thus, the current study aims to explore the differential expression of FAM26F in vitro in HepAD38 and HepG2 cell lines upon HBV infection, and in vivo in HBV infected individuals. The effects of antioxidant and calcium inhibitors on the regulation of FAM26F expression were also evaluated. The expression of FAM26F was simultaneously determined with well-established HBV infection markers: IRF3, and IFN-β. Methods The expression of FAM26F and marker genes was analyzed through Real-time qPCR and western blot. Results Our results indicate that the differential expression of FAM26F followed the same trend as that of IRF3 and IFN-β. The in vitro study revealed that, in both HBV infected cell lines, FAM26F expression was significantly down-regulated as compared to uninfected control cells. Treatment of cells with N-acetyl-L-cysteine (NAC), EGTA-AM, BAPTA-AM, and Ru360 significantly upregulated the expression of FAM26F in both the cell lines. Moreover, in in vivo study, FAM26F expression was significantly downregulated in all HBV infected groups as compared to controls (p = 0.0007). The expression was higher in the HBV recovered cases, probably due to the decrease in infection and increase in the immunity of these individuals. Conclusion Our study is the first to show the association of FAM26F with HBV infection. It is proposed that FAM26F expression could be an early predictive marker for HBV infection, and thus is worthy of further investigation. |
| Databáze: | OpenAIRE |
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