Ionizing radiation does not impair the mechanisms controlling genetic stability during T cell receptor gene rearrangement in mice
| Autor: | Simon Bouffler, Dag M. Eide, Ann-Karin Olsen, Serge M. Candéias, Dag Anders Brede, Sylwia Kabacik, Christophe Badie |
|---|---|
| Přispěvatelé: | Laboratoire de Chimie et Biologie des Métaux (LCBM - UMR 5249), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Public Health England [London], Centre for Radiation, Chemical and Environmental Hazards, Centre for Environmental Radioactivity (CERAD CoE), Department of Molecular Biology , Norwegian Institute of Public Health, Norwegian University of Life Sciences (NMBU), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male DNA damage Ataxia Telangiectasia Mutated Proteins DNA damage response Genomic Instability Ionizing radiation DNA Glycosylases 03 medical and health sciences Mice Radiation Ionizing Gene duplication Animals Radiology Nuclear Medicine and imaging [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Lymphocytes Receptor Gene low dose/low dose rate radiation Gene Rearrangement Radiological and Ultrasound Technology Chemistry X-Rays V(D)J recombination Wild type genetic instability Gene rearrangement T cell receptor genes Molecular biology Genes T-Cell Receptor 030104 developmental biology trans-rearrangements [SDV.IMM.IA]Life Sciences [q-bio]/Immunology/Adaptive immunology ATM Mice Inbred CBA |
| Zdroj: | International Journal of Radiation Biology International Journal of Radiation Biology, Informa Healthcare, 2018, 94 (4), pp.357-365. ⟨10.1080/09553002.2018.1439195⟩ International Journal of Radiation Biology, 2018, 94 (4), pp.357-365. ⟨10.1080/09553002.2018.1439195⟩ |
| ISSN: | 0955-3002 1362-3095 |
| DOI: | 10.1080/09553002.2018.1439195⟩ |
| Popis: | International audience; PURPOSE:To determine whether low dose/low dose rate radiation-induced genetic instability may result from radiation-induced inactivation of mechanisms induced by the ATM-dependent DNA damage response checkpoint. To this end, we analysed the faithfulness of T cell receptor (TR) gene rearrangement by V(D)J recombination in DNA from mice exposed to a single dose of X-ray or chronically exposed to low dose rate γ radiation.MATERIALS AND METHODS:Genomic DNA obtained from the blood or the thymus of wild type or Ogg1-deficient mice exposed to low (0.1) or intermediate/high (0.2-1 Gy) doses of radiation either by acute X-rays exposure or protracted exposure to low dose-rate γ-radiation was used to analyse by PCR the presence of illegitimate TR gene rearrangements.RESULTS:Radiation exposure does not increase the onset of TR gene trans-rearrangements in irradiated mice. In mice where it happens, trans-rearrangements remain sporadic events in developing T lymphocytes.CONCLUSION:We concluded that low dose/low dose rate ionizing radiation (IR) exposure does not lead to widespread inactivation of ATM-dependent mechanisms, and therefore that the mechanisms enforcing genetic stability are not impaired by IR in developing lymphocytes and lymphocyte progenitors, including BM-derived hematopoietic stem cells, in low dose/low dose rate exposed mice. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|