Deployment of a Prototype Plant GFP Imager at the Arthur Clarke Mars Greenhouse of the Haughton Mars Project
| Autor: | Anna-Lisa Paul, Trevor Murdoch, Thomas Graham, Matthew Bamsey, Robert J. Ferl, Alain Berinstain, Stephen Braham, Philip Neron |
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| Rok vydání: | 2008 |
| Předmět: |
Computer science
Transgene astrobiology Mars Greenhouse lcsh:Chemical technology Green Fluorescent Protein Biochemistry Full Research Paper analog environments Plant life Analytical Chemistry Green fluorescent protein Gene expression level lcsh:TP1-1185 Electrical and Electronic Engineering Instrumentation business.industry telemetry Mars Exploration Program Fluorescence Atomic and Molecular Physics and Optics Software deployment Systems engineering Extraterrestrial Environment Telecommunications business Biosensor |
| Zdroj: | Sensors; Volume 8; Issue 4; Pages: 2762-2773 Sensors (Basel, Switzerland) Sensors, Vol 8, Iss 4, Pp 2762-2773 (2008) |
| ISSN: | 1424-8220 |
| DOI: | 10.3390/s8042762 |
| Popis: | The use of engineered plants as biosensors has made elegant strides in the past decades, providing keen insights into the health of plants in general and particularly in the nature and cellular location of stress responses. However, most of the analytical procedures involve laboratory examination of the biosensor plants. With the advent of the green fluorescence protein (GFP) as a biosensor molecule, it became at least theoretically possible for analyses of gene expression to occur telemetrically, with the gene expression information of the plant delivered to the investigator over large distances simply as properly processed fluorescence images. Spaceflight and other extraterrestrial environments provide unique challenges to plant life, challenges that often require changes at the gene expression level to accommodate adaptation and survival. Having previously deployed transgenic plant biosensors to evaluate responses to orbital spaceflight, we wished to develop the plants and especially the imaging devices required to conduct such experiments robotically, without operator intervention, within extraterrestrial environments. This requires the development of an autonomous and remotely operated plant GFP imaging system and concomitant development of the communications infrastructure to manage dataflow from the imaging device. Here we report the results of deploying a prototype GFP imaging system within the Arthur Clarke Mars Greenhouse (ACMG) an autonomously operated greenhouse located within the Haughton Mars Project in the Canadian High Arctic. Results both demonstrate the applicability of the fundamental GFP biosensor technology and highlight the difficulties in collecting and managing telemetric data from challenging deployment environments. |
| Databáze: | OpenAIRE |
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