Wolbachiamodulates prevalence and viral load of Culex pipiens densoviruses in natural populations
Autor: | Mylène Weill, Mathieu Sicard, Fabienne Justy, Célestine M. Atyame, Mine Altinli, Sebastian Lequime |
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Přispěvatelé: | Lequime lab, Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UR226, Bernhard Nocht Institute for Tropical Medicine - Bernhard-Nocht-Institut für Tropenmedizin [Hamburg, Germany] (BNITM), German Centre for Infection Research (DZIF), Groningen Institute for Evolutionary Life Sciences [Groningen] (GELIFES), University of Groningen [Groningen], Processus Infectieux en Milieu Insulaire Tropical (PIMIT), Centre National de la Recherche Scientifique (CNRS)-IRD-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de La Réunion (UR), ANR-16-CE02-0006,CIAWOL,Bases moléculaire et cellulaire de la diversité phénotypique de l'incompatibilté cytoplasmique chez les insectes(2016), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS), Université de La Réunion (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IRD-Centre National de la Recherche Scientifique (CNRS), Herrada, Anthony, Bases moléculaire et cellulaire de la diversité phénotypique de l'incompatibilté cytoplasmique chez les insectes - - CIAWOL2016 - ANR-16-CE02-0006 - AAPG2016 - VALID, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École pratique des hautes études (EPHE) |
Rok vydání: | 2020 |
Předmět: |
0106 biological sciences
0301 basic medicine 010603 evolutionary biology 01 natural sciences densovirus 03 medical and health sciences insect‐specific virus Phylogenetics Culex pipiens parasitic diseases Prevalence Genetics Animals Phylogeny reproductive and urinary physiology Ecology Evolution Behavior and Systematics [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology biology Phylogenetic tree [SDV.EE.IEO] Life Sciences [q-bio]/Ecology environment/Symbiosis Viral Load biochemical phenomena metabolism and nutrition biology.organism_classification 3. Good health Culex Densovirinae 030104 developmental biology [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Horizontal gene transfer microbiota interactions bacteria Wolbachia Densovirus Cytoplasmic incompatibility [SDV.EE.IEO]Life Sciences [q-bio]/Ecology environment/Symbiosis |
Zdroj: | Molecular Ecology, 29(20), 4000-4013. Wiley Molecular Ecology Molecular Ecology, Wiley, In press, ⟨10.1111/mec.15609⟩ Molecular Ecology, In press, ⟨10.1111/mec.15609⟩ |
ISSN: | 1365-294X 0962-1083 |
Popis: | International audience; The inadequacy of standard mosquito control strategies calls for ecologically safe novel approaches, for example the use of biological agents such as the endosymbiotic α‐proteobacteria Wolbachia or insect‐specific viruses (ISVs). Understanding the ecological interactions between these “biocontrol endosymbionts” is thus a fundamental step. Wolbachia are transmitted vertically from mother to offspring and modify their hosts’ phenotypes, including reproduction (e.g., cytoplasmic incompatibility) and survival (e.g., viral interference). In nature, Culex pipiens (sensu lato) mosquitoes are always found infected with genetically diverse Wolbachia called wPip that belong to five phylogenetic groups. In recent years, ISVs have also been discovered in these mosquito species, although their interactions with Wolbachia in nature are unknown. Here, we studied the interactions between a widely prevalent ISV, the Culex pipiens densovirus (CpDV, Densovirinae), and Wolbachia in northern Tunisian C. pipiens populations. We showed an influence of different Wolbachia groups on CpDV prevalence and a general positive correlation between Wolbachia and CpDV loads. By investigating the putative relationship between CpDV diversification and wPip groups in the different sites, we detected a signal linked to wPip groups in CpDV phylogeny in sites where all larvae were infected by the same wPip group. However, no such signal was detected where the wPip groups coexisted, suggesting CpDV horizontal transfer between hosts. Overall, our results provide good evidence for an ecological influence of Wolbachia on an ISV, CpDV, in natural populations and highlight the importance of integrating Wolbachia in our understanding of ISV ecology in nature. |
Databáze: | OpenAIRE |
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