Wolbachiamodulates prevalence and viral load of Culex pipiens densoviruses in natural populations

Autor: Mylène Weill, Mathieu Sicard, Fabienne Justy, Célestine M. Atyame, Mine Altinli, Sebastian Lequime
Přispěvatelé: Lequime lab, Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UR226, Bernhard Nocht Institute for Tropical Medicine - Bernhard-Nocht-Institut für Tropenmedizin [Hamburg, Germany] (BNITM), German Centre for Infection Research (DZIF), Groningen Institute for Evolutionary Life Sciences [Groningen] (GELIFES), University of Groningen [Groningen], Processus Infectieux en Milieu Insulaire Tropical (PIMIT), Centre National de la Recherche Scientifique (CNRS)-IRD-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de La Réunion (UR), ANR-16-CE02-0006,CIAWOL,Bases moléculaire et cellulaire de la diversité phénotypique de l'incompatibilté cytoplasmique chez les insectes(2016), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS), Université de La Réunion (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IRD-Centre National de la Recherche Scientifique (CNRS), Herrada, Anthony, Bases moléculaire et cellulaire de la diversité phénotypique de l'incompatibilté cytoplasmique chez les insectes - - CIAWOL2016 - ANR-16-CE02-0006 - AAPG2016 - VALID, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École pratique des hautes études (EPHE)
Rok vydání: 2020
Předmět:
0106 biological sciences
0301 basic medicine
010603 evolutionary biology
01 natural sciences
densovirus
03 medical and health sciences
insect‐specific virus
Phylogenetics
Culex pipiens
parasitic diseases
Prevalence
Genetics
Animals
Phylogeny
reproductive and urinary physiology
Ecology
Evolution
Behavior and Systematics
[SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology
biology
Phylogenetic tree
[SDV.EE.IEO] Life Sciences [q-bio]/Ecology
environment/Symbiosis
Viral Load
biochemical phenomena
metabolism
and nutrition
biology.organism_classification
3. Good health
Culex
Densovirinae
030104 developmental biology
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Horizontal gene transfer
microbiota interactions
bacteria
Wolbachia
Densovirus
Cytoplasmic incompatibility
[SDV.EE.IEO]Life Sciences [q-bio]/Ecology
environment/Symbiosis
Zdroj: Molecular Ecology, 29(20), 4000-4013. Wiley
Molecular Ecology
Molecular Ecology, Wiley, In press, ⟨10.1111/mec.15609⟩
Molecular Ecology, In press, ⟨10.1111/mec.15609⟩
ISSN: 1365-294X
0962-1083
Popis: International audience; The inadequacy of standard mosquito control strategies calls for ecologically safe novel approaches, for example the use of biological agents such as the endosymbiotic α‐proteobacteria Wolbachia or insect‐specific viruses (ISVs). Understanding the ecological interactions between these “biocontrol endosymbionts” is thus a fundamental step. Wolbachia are transmitted vertically from mother to offspring and modify their hosts’ phenotypes, including reproduction (e.g., cytoplasmic incompatibility) and survival (e.g., viral interference). In nature, Culex pipiens (sensu lato) mosquitoes are always found infected with genetically diverse Wolbachia called wPip that belong to five phylogenetic groups. In recent years, ISVs have also been discovered in these mosquito species, although their interactions with Wolbachia in nature are unknown. Here, we studied the interactions between a widely prevalent ISV, the Culex pipiens densovirus (CpDV, Densovirinae), and Wolbachia in northern Tunisian C. pipiens populations. We showed an influence of different Wolbachia groups on CpDV prevalence and a general positive correlation between Wolbachia and CpDV loads. By investigating the putative relationship between CpDV diversification and wPip groups in the different sites, we detected a signal linked to wPip groups in CpDV phylogeny in sites where all larvae were infected by the same wPip group. However, no such signal was detected where the wPip groups coexisted, suggesting CpDV horizontal transfer between hosts. Overall, our results provide good evidence for an ecological influence of Wolbachia on an ISV, CpDV, in natural populations and highlight the importance of integrating Wolbachia in our understanding of ISV ecology in nature.
Databáze: OpenAIRE
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