Evidence for a role of FGF-2 and FGF receptors in the proliferation of non-small cell lung cancer cells
| Autor: | F. Eckersberger, Ulrike Setinek, Carlos Caldas, Walter Berger, Gerhard Dekan, Thomas Mohr, Michael Micksche, Ingela Kindås-Mügge, Monika Vetterlein |
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| Rok vydání: | 1999 |
| Předmět: |
Cancer Research
medicine.medical_specialty Lung Neoplasms medicine.medical_treatment Biology Fibroblast growth factor Mice Paracrine signalling Cell surface receptor Carcinoma Non-Small-Cell Lung Internal medicine Tumor Cells Cultured medicine Animals Humans RNA Messenger Autocrine signalling Cell growth Growth factor 3T3 Cells Receptors Fibroblast Growth Factor Recombinant Proteins respiratory tract diseases Cytokine Endocrinology Oncology Fibroblast growth factor receptor Cancer research Cattle Fibroblast Growth Factor 2 Cell Division |
| Zdroj: | International Journal of Cancer. 83:415-423 |
| ISSN: | 1097-0215 0020-7136 |
| Popis: | Basic fibroblast growth factor (FGF-2) has been implicated in the progression of human tumours via both autocrine and paracrine (angiogenic) activities. We investigated the expression of FGF-2 and FGF receptors (FGFR-1 to -4) in NSCLC cell lines (N = 16), NSCLC surgical specimens (N = 21) and 2 control cell lines. Our data show that almost all NSCLC cells produce elevated levels of FGF-2 and FGFR in vitro and in vivo. FGF-2 expression did correlate with a short doubling time as well as with potent anchorage-independent growth of NSCLC cell lines. In contrast with control cells, NSCLC cells did not secrete considerable amounts of FGF-2 into the extracellular space. Expression levels of FGFR-1 and -2 in NSCLC cell lines correlated with FGF-2 production. FGFR were located at the plasma membranes in some low FGF-2-producing NSCLC and control cell lines. These cells were sensitive to the proliferative effect of recombinant FGF-2 (rFGF-2). In NSCLC cell lines with an enhanced FGF-2 production, representing the majority studied, FGFR localisation was predominantly intracellular. These cells were insensitive to both the proliferative effect of rFGF-2 and growth inhibition by FGF-2-neutralising antibodies. In contrast, several agents antagonised FGF-2 intracellularly impaired growth of almost all NSCLC cell lines. Our data suggest a role of FGF-2 and FGFR in the growth stimulation of NSCLC cells possibly via an intracrine mechanism. |
| Databáze: | OpenAIRE |
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