Comparison of the Idaho Technology FilmArray system to real-time PCR for detection of respiratory pathogens in children
| Autor: | Richard L. Hodinka, Michael Elkan, Karin L. McGowan, Virginia M. Pierce, Marilyn Leet |
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| Rok vydání: | 2011 |
| Předmět: |
Microbiology (medical)
Serotype Male Adolescent Biology medicine.disease_cause Sensitivity and Specificity Salt lake Young Adult Virology Influenza A virus medicine Humans Child Respiratory Tract Infections Respiratory tract infections Infant Newborn Infant Nucleic acid amplification technique Respiratory pathogens Real-time polymerase chain reaction Molecular Diagnostic Techniques Virus Diseases Child Preschool Female Viral load Nucleic Acid Amplification Techniques |
| Zdroj: | Journal of Clinical Microbiology |
| ISSN: | 1098-660X |
| Popis: | The FilmArray Respiratory Panel (RP) multiplexed nucleic acid amplification test (Idaho Technology, Inc., Salt Lake City, UT) was compared to laboratory-developed real-time PCR assays for the detection of various respiratory viruses and certain bacterial pathogens. A total of 215 frozen archived pediatric respiratory specimens previously characterized as either negative or positive for one or more pathogens by real-time PCR were examined using the FilmArray RP system. Overall agreement between the FilmArray RP and corresponding real-time PCR assays for shared analytes was 98.6% (kappa = 0.92 [95% confidence interval (CI), 0.89 to 0.94]). The combined positive percent agreement was 89.4% (95% CI, 85.4 to 92.6); the negative percent agreement was 99.6% (95% CI, 99.2 to 99.8). The mean real-time PCR threshold cycle ( C T ) value for specimens with discordant results was 36.46 ± 4.54. Detection of coinfections and correct identification of influenza A virus subtypes were comparable to those of real-time PCR when using the FilmArray RP. The greatest comparative difference in sensitivity was observed for adenovirus; only 11 of 24 (45.8%; 95% CI, 27.9 to 64.9) clinical specimens positive for adenovirus by real-time PCR were also positive by the FilmArray RP. In addition, upon testing 20 characterized adenovirus serotypes prepared at high and low viral loads, the FilmArray RP did not detect serotypes 6 and 41 at either level and failed to detect serotypes 2, 20, 35, and 37 when viral loads were low. The FilmArray RP system is rapid and extremely user-friendly, with results available in just over 1 h with almost no labor involved. Its low throughput is a significant drawback for laboratories receiving large numbers of specimens, as only a single sample can be processed at a time with one instrument. |
| Databáze: | OpenAIRE |
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