Alternative activation of macrophage by IL-10
| Autor: | Manfred Kauer, Hubert Kolb, Stephan Martin, R. Stumpo |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
DNA
Complementary Macrophages Gene Expression Nucleic Acid Hybridization RNA-Binding Proteins Sequence Homology Cell Biology General Medicine Biology Macrophage Activation Poly(A)-Binding Proteins Polymerase Chain Reaction Pathology and Forensic Medicine Proinflammatory cytokine Cell Line Interleukin-10 Interleukin 10 Mice Differentially expressed genes Immunology Macrophage Animals RNA Messenger Cloning Molecular Molecular Biology |
| Zdroj: | Pathobiology : journal of immunopathology, molecular and cellular biology. 67(5-6) |
| ISSN: | 1015-2008 |
| Popis: | Macrophage activation by proinflammatory stimuli is suppressed by IL-10. We tested the hypothesis that IL-10 induces an alternative state of macrophage activation rather than solely mediating suppression. We therefore searched for genes the expression of which might be up-rather than downregulated in response to IL-10. Total RNA was obtained from mouse macrophages J774 A.1 before or after stimulation with IL-10 (20 ng/ml). Poly(A)+RNA was isolated in both cases in order to obtain driver and tester mRNA. Subtraction suppression hybridization was performed using the PCR-select cDNA subtraction procedure. After evaluation of the subtraction efficiency the subtracted cDNA library was cloned into pCRII.1. A total of 1,300 clones were obtained. Southern blot hybridization analysis was performed as the first screening step of this total number of clones. 140 (10.7%) were identified as upregulated in response to IL-10. Sequence analyses so far showed perfect or near perfect matches with already known genes for the majority of clones. Our results clearly indicate that IL-10 stimulates the expression of a large number of genes in macrophages. We conclude that IL-10 induces in macrophages a noninflammatory state of reactivity which may serve to contain proinflammatory conditions. |
| Databáze: | OpenAIRE |
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