Limited and excess protein intake of pregnant gilts differently affects body composition and cellularity of skeletal muscle and subcutaneous adipose tissue of newborn and weanling piglets

Autor: Ralf Pfuhl, Cornelia C. Metges, Jana Block, Bernd Stabenow, Claudia Kalbe, Louis Lefaucheur, Winfried Otten, Charlotte Rehfeldt
Přispěvatelé: Research Unit Muscle Biology and Growth, Leibniz Institute for Farm Animal Biology (FBN), Unité mixte de recherche veau et porc (UMR VP), Ecole Nationale Supérieure Agronomique de Rennes-Institut National de la Recherche Agronomique (INRA)
Rok vydání: 2011
Předmět:
Male
Muscle tissue
medicine.medical_specialty
Swine
Offspring
Subcutaneous Fat
Medicine (miscellaneous)
Weanling
Biology
Muscle Development
Body composition
Muscle hypertrophy
03 medical and health sciences
chemistry.chemical_compound
Pregnancy
Adipocyte
Internal medicine
Myosin
Adipocytes
medicine
Animals
Birth Weight
Myocyte
Muscle
Skeletal
Muscle fiber
030304 developmental biology
2. Zero hunger
Pig
0303 health sciences
Nutrition and Dietetics
0402 animal and dairy science
Skeletal muscle
Maternal Nutritional Physiological Phenomena
04 agricultural and veterinary sciences
Fetal development
Animal Feed
040201 dairy & animal science
Diet
Pregnancy protein intake
medicine.anatomical_structure
Endocrinology
Adipose Tissue
Animals
Newborn
chemistry
Animal Nutritional Physiological Phenomena
Female
Dietary Proteins
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Zdroj: European Journal of Nutrition
European Journal of Nutrition, Springer Verlag, 2012, 51 (2), pp.151-165. ⟨10.1007/s00394-011-0201-8⟩
ISSN: 1436-6215
1436-6207
Popis: International audience; This study investigated whether dietary protein intake less (50%) or greater (250%) than requirements throughout gestation differently affects offspring body composition and cellular properties of skeletal muscle and subcutaneous adipose tissue (SCAT). Primiparous gilts were fed iso-energetic diets containing adequate (22 AP), high (21 HP), or low (19 LP) protein contents. Newborn (n = 166) and weanling piglets cross-fostered to sows fed a standard diet (day 28; n = 83) were examined by morphological, biochemical, histological, and molecular analyses of the body, SCAT, and semitendinosus, longissimus, biceps femoris muscles. Lowered birth weight (BW) in response to the HP and LP diets (p < 0.01) resulted from decreases in all body constituents in LP, and mainly from reduced body fat in HP piglets (p < 0.05). In the light BW class within litters, HP piglets exhibited a greater percentage of muscle tissue (p < 0.05) than LP piglets. Less SCAT mass in HP and LP piglets resulted from reduced (p < 0.05) number, but not the size of adipocytes. The LP diet adversely affected myogenesis and muscular differentiation derived from less (p < 0.01) primary and secondary myofibers, lower creatine kinase activity (p < 0.05), less IGF2 mRNA (p < 0.10), and greater expression of the embryonic myosin heavy chain isoform (p < 0.01). Catch-up growth of LP but not HP pigs until day 28 increased body fat (p = 0.01). Despite compensated muscle growth in LP piglets, the deficit in myofiber number remained. Poor intrauterine environment by limited and excess protein supply retards fetal growth, but only limited protein supply impairs myogenesis, persistently restricts muscle growth potential, and favors obesity at infancy.
Databáze: OpenAIRE
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