A comparison of sexing methods in fetal mice
| Autor: | Scott Deeney, Kyle N Powers, Timothy M. Crombleholme |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Sex Determination Analysis Gonad Genotyping Techniques Sexing Biology Andrology 03 medical and health sciences 0302 clinical medicine medicine Animals Genitalia Genes sry Genotyping Fetus Sex Characteristics General Veterinary Pigmentation Fetal period Reproducibility of Results Embryo Gold standard (test) Embryo Mammalian Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Testis determining factor Animal Science and Zoology Female 030217 neurology & neurosurgery |
| Zdroj: | Lab animal. 45(10) |
| ISSN: | 1548-4475 |
| Popis: | Accurate mouse sexing is vital when conducting research examining sexual dimorphisms. Late fetal and newborn mouse pups are more immature than many previously described sexing methods allow. This study compares the sexing accuracy of a newly described internal gonad sexing method to a recently described peritoneal pigmentation sexing method in embryonic day 20 C57BL/6J mouse pups, using Sry genotyping to confirm the sex. The internal gonad sexing method was found to be highly accurate, while the peritoneal pigmentation method was slightly less accurate. Therefore, while Sry genotyping remains the gold standard, immediate and less expensive sexing methods can be performed accurately as early as the late fetal period in C57BL/6J mice. |
| Databáze: | OpenAIRE |
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