Atorvastatin and Caffeine in Combination Regulates Apoptosis, Migration, Invasion and Tumorspheres of Prostate Cancer Cells
| Autor: | Hongping Xiang, Yan He, Zheng Wan, Xi Zheng, Kun Zhang, Wang Zhenshi, Huarong Huang, Yang Liu, Wu Xiaofeng, Zhiyun Du, Lanyue Zhang, Susan Goodin |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Cancer Research Statin Phosphodiesterase Inhibitors medicine.drug_class Atorvastatin Apoptosis Pathology and Forensic Medicine 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cell Movement Caffeine Survivin medicine Humans Cell Proliferation Chemistry Cell growth Prostatic Neoplasms Cell migration General Medicine 030104 developmental biology Oncology 030220 oncology & carcinogenesis PC-3 Cells Cancer cell Cancer research Trypan blue Hydroxymethylglutaryl-CoA Reductase Inhibitors medicine.drug |
| Zdroj: | Pathology & Oncology Research. 26:209-216 |
| ISSN: | 1532-2807 1219-4956 |
| DOI: | 10.1007/s12253-018-0415-7 |
| Popis: | Atorvastatin is the most prescribed cholesterol-lowering statin, while caffeine enhances chemo-sensitivity and induces apoptosis of tumor cells through its DNA repair-inhibiting effect. The present study investigated the effects and mechanisms of atorvastatin and caffeine in combination on human prostate cancer cells cultured in vitro. Cell growth were determined by the trypan blue exclusion assay. The cell apoptosis and colony formation were determined by morphological assessment. The ability of cell migration and invasion were performed using a scratch wound-healing and Transwell assay. Tumorspheres were formed in suspension under the condition of non-adherence and serum-free medium. Finally, the western blot assay was used to determine the levels of proteins. The combination synergistically suppressed proliferation and induced apoptotic death. Meanwhile, the migration, invasion, and the formation of tumorspheres were significantly inhibited by the combination. We found that atorvastatin and caffeine in combination downregulated phospho-Akt, phospho-Erk1/2, anti-apoptotic Bcl-2 and Survivin protein levels. Results of the present study indicate treatment with the combination of caffeine and atorvastatin may be an effective strategy for inhibiting the growth of prostate cancer and should be evaluated clinically. |
| Databáze: | OpenAIRE |
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